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Prevention of ultraviolet radiation-induced suppression of contact and delayed hypersensitivity by Aloe barbadensis gel extract.
J Invest Dermatol. 1994 Feb; 102(2):197-204.JI

Abstract

We investigated the ability of Aloe barbadensis gel extract to prevent suppression of contact hypersensitivity (CHS) and delayed-type hypersensitivity (DTH) responses in mice by ultraviolet (UV) irradiation. Local immune suppression was induced in C3H mice by exposure to four daily doses of 400 J/m2 UV-B (280-320 nm) radiation from FS40 sunlamps, followed by sensitization with 0.5% fluorescein isothiocyanate (FITC) through the irradiated skin. Topical application of 0.167-1.67% Aloe gel after each irradiation significantly reduced this suppression. Aloe treatment partially preserved the number and morphology of Langerhans and Thy-1+ dendritic epidermal cells in skin, compared to those in the skin of mice given only UVR or UVR plus the vehicle. Experiments using a single (2 kJ/m2) dose of UVR followed by Aloe treatment showed that the effect of Aloe was not due to screening of the UVR. Systemic suppression of DTH to Candida albicans or CHS to FITC was induced in C3H mice exposed to 5 or 10 kJ/m2 UV-B radiation, respectively, on shaved dorsal skin and sensitized 3 d later with a subcutaneous injection of formalin-fixed Candida or FITC painted on unirradiated, ventral skin. Treatment of the UV-irradiated skin with Aloe immediately after irradiation prevented suppression of both DTH to Candida and CHS to FITC. Aloe treatment did not prevent the formation of cyclobutyl pyrimidine dimers in the DNA of UV-irradiated skin or accelerate the repair of these lesions. These studies demonstrate that topical application of Aloe barbadensis gel extract to the skin of UV-irradiated mice ameliorates UV-induced immune suppression by a mechanism that does not involve DNA damage or repair.

Links

Publisher Full Text
linkinghub.elsevier.com
linkinghub.elsevier.com

Authors+Show Affiliations

Strickland FM
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Pelley RP
No affiliation info available
Kripke ML
No affiliation info available

MeSH

Adenosine TriphosphatasesAdministration, TopicalAloeAnimalsAntigens, SurfaceCandida albicansDNADNA DamageDendritic CellsDermatitis, ContactDose-Response Relationship, RadiationFemaleFluorescein-5-isothiocyanateGelsHistocompatibility Antigens Class IIHypersensitivity, DelayedImmunosuppressionLangerhans CellsMembrane GlycoproteinsMiceMice, Inbred C3HPlant ExtractsPlants, MedicinalRadiation Injuries, ExperimentalSkinSunscreening AgentsThy-1 AntigensTime FactorsUltraviolet Rays

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7906286

Citation

Strickland, F M., et al. "Prevention of Ultraviolet Radiation-induced Suppression of Contact and Delayed Hypersensitivity By Aloe Barbadensis Gel Extract." The Journal of Investigative Dermatology, vol. 102, no. 2, 1994, pp. 197-204.
Strickland FM, Pelley RP, Kripke ML. Prevention of ultraviolet radiation-induced suppression of contact and delayed hypersensitivity by Aloe barbadensis gel extract. J Invest Dermatol. 1994;102(2):197-204.
Strickland, F. M., Pelley, R. P., & Kripke, M. L. (1994). Prevention of ultraviolet radiation-induced suppression of contact and delayed hypersensitivity by Aloe barbadensis gel extract. The Journal of Investigative Dermatology, 102(2), 197-204.
Strickland FM, Pelley RP, Kripke ML. Prevention of Ultraviolet Radiation-induced Suppression of Contact and Delayed Hypersensitivity By Aloe Barbadensis Gel Extract. J Invest Dermatol. 1994;102(2):197-204. PubMed PMID: 7906286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevention of ultraviolet radiation-induced suppression of contact and delayed hypersensitivity by Aloe barbadensis gel extract. AU - Strickland,F M, AU - Pelley,R P, AU - Kripke,M L, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 197 EP - 204 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 102 IS - 2 N2 - We investigated the ability of Aloe barbadensis gel extract to prevent suppression of contact hypersensitivity (CHS) and delayed-type hypersensitivity (DTH) responses in mice by ultraviolet (UV) irradiation. Local immune suppression was induced in C3H mice by exposure to four daily doses of 400 J/m2 UV-B (280-320 nm) radiation from FS40 sunlamps, followed by sensitization with 0.5% fluorescein isothiocyanate (FITC) through the irradiated skin. Topical application of 0.167-1.67% Aloe gel after each irradiation significantly reduced this suppression. Aloe treatment partially preserved the number and morphology of Langerhans and Thy-1+ dendritic epidermal cells in skin, compared to those in the skin of mice given only UVR or UVR plus the vehicle. Experiments using a single (2 kJ/m2) dose of UVR followed by Aloe treatment showed that the effect of Aloe was not due to screening of the UVR. Systemic suppression of DTH to Candida albicans or CHS to FITC was induced in C3H mice exposed to 5 or 10 kJ/m2 UV-B radiation, respectively, on shaved dorsal skin and sensitized 3 d later with a subcutaneous injection of formalin-fixed Candida or FITC painted on unirradiated, ventral skin. Treatment of the UV-irradiated skin with Aloe immediately after irradiation prevented suppression of both DTH to Candida and CHS to FITC. Aloe treatment did not prevent the formation of cyclobutyl pyrimidine dimers in the DNA of UV-irradiated skin or accelerate the repair of these lesions. These studies demonstrate that topical application of Aloe barbadensis gel extract to the skin of UV-irradiated mice ameliorates UV-induced immune suppression by a mechanism that does not involve DNA damage or repair. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/7906286/Prevention_of_ultraviolet_radiation_induced_suppression_of_contact_and_delayed_hypersensitivity_by_Aloe_barbadensis_gel_extract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(94)97757-7 DB - PRIME DP - Unbound Medicine ER -