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Synthesis and release of amphipathic gamma-glutamyl transferase by the pulmonary alveolar type 2 cell. Its redistribution throughout the gas exchange portion of the lung indicates a new role for surfactant.
J Biol Chem 1994; 269(19):14219-26JB

Abstract

gamma-Glutamyl transferase (gamma-GT) catalyzes a transpeptidation reaction which is involved in the metabolism of glutathione. Glutathione is abundant within the epithelial lining fluid of the lung. However, little is known about gamma-GT expression in the epithelial cells of the lung alveolus. Herein we show that the pulmonary alveolar epithelial type 2 cell expresses the gene for gamma-GT. We were unable to detect expression in the pulmonary alveolar epithelial type 1 cell or in the pulmonary alveolar macrophage. gamma-GT expression in the pulmonary alveolar epithelial type 2 cell is via mRNA III, a transcript that was initially cloned from the liver. This cell synthesizes gamma-GT protein and releases enzyme activity into a surfactant-associated pool within the lung alveolus. The specific activity of this surfactant-associated enzyme is almost 10-fold higher than that of whole lung. This activity results from amphipathic gamma-GT since it partitions with lung surfactant phospholipid and with the detergent phase of Triton X-114. Activity can be dissociated from each by papain proteolysis. These results demonstrate that gamma-GT is expressed in the differentiated pulmonary alveolar epithelial type 2 cell and that amphipathic gamma-GT protein is released by this cell along with lung surfactant. These results suggest that surfactant may serve an expanded role in lung cell biology as the vehicle for the redistribution of amphipathic signal anchored proteins throughout the gas exchange surface of the lung.

Authors+Show Affiliations

Pulmonary Center, Boston University School of Medicine, Massachusetts 02118.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7910609

Citation

Joyce-Brady, M, et al. "Synthesis and Release of Amphipathic Gamma-glutamyl Transferase By the Pulmonary Alveolar Type 2 Cell. Its Redistribution Throughout the Gas Exchange Portion of the Lung Indicates a New Role for Surfactant." The Journal of Biological Chemistry, vol. 269, no. 19, 1994, pp. 14219-26.
Joyce-Brady M, Takahashi Y, Oakes SM, et al. Synthesis and release of amphipathic gamma-glutamyl transferase by the pulmonary alveolar type 2 cell. Its redistribution throughout the gas exchange portion of the lung indicates a new role for surfactant. J Biol Chem. 1994;269(19):14219-26.
Joyce-Brady, M., Takahashi, Y., Oakes, S. M., Rishi, A. K., Levine, R. A., Kinlough, C. L., & Hughey, R. P. (1994). Synthesis and release of amphipathic gamma-glutamyl transferase by the pulmonary alveolar type 2 cell. Its redistribution throughout the gas exchange portion of the lung indicates a new role for surfactant. The Journal of Biological Chemistry, 269(19), pp. 14219-26.
Joyce-Brady M, et al. Synthesis and Release of Amphipathic Gamma-glutamyl Transferase By the Pulmonary Alveolar Type 2 Cell. Its Redistribution Throughout the Gas Exchange Portion of the Lung Indicates a New Role for Surfactant. J Biol Chem. 1994 May 13;269(19):14219-26. PubMed PMID: 7910609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and release of amphipathic gamma-glutamyl transferase by the pulmonary alveolar type 2 cell. Its redistribution throughout the gas exchange portion of the lung indicates a new role for surfactant. AU - Joyce-Brady,M, AU - Takahashi,Y, AU - Oakes,S M, AU - Rishi,A K, AU - Levine,R A, AU - Kinlough,C L, AU - Hughey,R P, PY - 1994/5/13/pubmed PY - 1994/5/13/medline PY - 1994/5/13/entrez SP - 14219 EP - 26 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 269 IS - 19 N2 - gamma-Glutamyl transferase (gamma-GT) catalyzes a transpeptidation reaction which is involved in the metabolism of glutathione. Glutathione is abundant within the epithelial lining fluid of the lung. However, little is known about gamma-GT expression in the epithelial cells of the lung alveolus. Herein we show that the pulmonary alveolar epithelial type 2 cell expresses the gene for gamma-GT. We were unable to detect expression in the pulmonary alveolar epithelial type 1 cell or in the pulmonary alveolar macrophage. gamma-GT expression in the pulmonary alveolar epithelial type 2 cell is via mRNA III, a transcript that was initially cloned from the liver. This cell synthesizes gamma-GT protein and releases enzyme activity into a surfactant-associated pool within the lung alveolus. The specific activity of this surfactant-associated enzyme is almost 10-fold higher than that of whole lung. This activity results from amphipathic gamma-GT since it partitions with lung surfactant phospholipid and with the detergent phase of Triton X-114. Activity can be dissociated from each by papain proteolysis. These results demonstrate that gamma-GT is expressed in the differentiated pulmonary alveolar epithelial type 2 cell and that amphipathic gamma-GT protein is released by this cell along with lung surfactant. These results suggest that surfactant may serve an expanded role in lung cell biology as the vehicle for the redistribution of amphipathic signal anchored proteins throughout the gas exchange surface of the lung. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/7910609/Synthesis_and_release_of_amphipathic_gamma_glutamyl_transferase_by_the_pulmonary_alveolar_type_2_cell__Its_redistribution_throughout_the_gas_exchange_portion_of_the_lung_indicates_a_new_role_for_surfactant_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=7910609 DB - PRIME DP - Unbound Medicine ER -