Tags

Type your tag names separated by a space and hit enter

The effect of nimodipine on cochlear potentials and Na+/K(+)-ATPase activity in normal and hydropic cochleas of the albino guinea pig.
Hear Res. 1994 Jun 15; 77(1-2):9-18.HR

Abstract

In experimental endolymphatic hydrops (EEH) a decrease in the endocochlear potential (EP) has been reported and is thought to be due to decreased activity of the enzyme Na+/K(+)-ATPase in the stria vascularis. By stimulating Na+/K(+)-ATPase, the EP, and thereby cochlear function as a whole, might be restored. On the other hand, stimulation of stria vascularis Na+/K(+)-ATPase might result in excessive production of endolymph and thus produce or augment hydrops. In this study we have investigated the effect of intraperitoneally applied nimodipine on cochlear potentials and on Na+/K(+)-ATPase activity in the stria vascularis, both in normal cochleas (control) and in cochleas with EEH. Nimodipine is an L-type Ca(2+)-channel blocking agent with Na+/K(+)-ATPase stimulating properties at concentrations as low as 1.5 nM. The compound action potential (CAP), evoked by 2,4 and 8 kHz tone bursts was found to be depressed in the EEH ears with and without nimodipine treatment, and in the nimodipine treated control ears. Statistical analysis (ANOVA) showed that the effects of EEH and nimodipine on the CAP were additive. The negative summating potential (SP), measured extracochlearly at the apex, in response to 4 and 8 kHz tone bursts was significantly enhanced in the EEH ears. Nimodipine treatment did not affect the SP, neither in the control, nor in the EEH ears. Cytochemically, Na+/K(+)-ATPase activity appeared to be decreased in the oedematous stria vascularis of hydropic cochleas. No effect of nimodipine on Na+/K(+)-ATPase activity could be established ultracytochemically, neither in the controls nor in the EEH ears. In the lower turns of some of the nimodipine treated control cochleas a mild hydrops was seen during light-microscopic evaluation. Although it was not possible to prove a stimulatory effect of nimodipine on the enzyme Na+/K(+)-ATPase cytochemically, the finding of mild endolymphatic hydrops in nimodipine treated control ears suggests (a history of) increased endolymph production. This hydrops might be responsible for the depression of the CAP in the nimodipine treated ears.

Authors+Show Affiliations

Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7928742

Citation

van Benthem, P P., et al. "The Effect of Nimodipine On Cochlear Potentials and Na+/K(+)-ATPase Activity in Normal and Hydropic Cochleas of the Albino Guinea Pig." Hearing Research, vol. 77, no. 1-2, 1994, pp. 9-18.
van Benthem PP, Klis SF, Albers FW, et al. The effect of nimodipine on cochlear potentials and Na+/K(+)-ATPase activity in normal and hydropic cochleas of the albino guinea pig. Hear Res. 1994;77(1-2):9-18.
van Benthem, P. P., Klis, S. F., Albers, F. W., de Wildt, D. J., Veldman, J. E., Huizing, E. H., & Smoorenburg, G. F. (1994). The effect of nimodipine on cochlear potentials and Na+/K(+)-ATPase activity in normal and hydropic cochleas of the albino guinea pig. Hearing Research, 77(1-2), 9-18.
van Benthem PP, et al. The Effect of Nimodipine On Cochlear Potentials and Na+/K(+)-ATPase Activity in Normal and Hydropic Cochleas of the Albino Guinea Pig. Hear Res. 1994 Jun 15;77(1-2):9-18. PubMed PMID: 7928742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of nimodipine on cochlear potentials and Na+/K(+)-ATPase activity in normal and hydropic cochleas of the albino guinea pig. AU - van Benthem,P P, AU - Klis,S F, AU - Albers,F W, AU - de Wildt,D J, AU - Veldman,J E, AU - Huizing,E H, AU - Smoorenburg,G F, PY - 1994/6/15/pubmed PY - 1994/6/15/medline PY - 1994/6/15/entrez SP - 9 EP - 18 JF - Hearing research JO - Hear Res VL - 77 IS - 1-2 N2 - In experimental endolymphatic hydrops (EEH) a decrease in the endocochlear potential (EP) has been reported and is thought to be due to decreased activity of the enzyme Na+/K(+)-ATPase in the stria vascularis. By stimulating Na+/K(+)-ATPase, the EP, and thereby cochlear function as a whole, might be restored. On the other hand, stimulation of stria vascularis Na+/K(+)-ATPase might result in excessive production of endolymph and thus produce or augment hydrops. In this study we have investigated the effect of intraperitoneally applied nimodipine on cochlear potentials and on Na+/K(+)-ATPase activity in the stria vascularis, both in normal cochleas (control) and in cochleas with EEH. Nimodipine is an L-type Ca(2+)-channel blocking agent with Na+/K(+)-ATPase stimulating properties at concentrations as low as 1.5 nM. The compound action potential (CAP), evoked by 2,4 and 8 kHz tone bursts was found to be depressed in the EEH ears with and without nimodipine treatment, and in the nimodipine treated control ears. Statistical analysis (ANOVA) showed that the effects of EEH and nimodipine on the CAP were additive. The negative summating potential (SP), measured extracochlearly at the apex, in response to 4 and 8 kHz tone bursts was significantly enhanced in the EEH ears. Nimodipine treatment did not affect the SP, neither in the control, nor in the EEH ears. Cytochemically, Na+/K(+)-ATPase activity appeared to be decreased in the oedematous stria vascularis of hydropic cochleas. No effect of nimodipine on Na+/K(+)-ATPase activity could be established ultracytochemically, neither in the controls nor in the EEH ears. In the lower turns of some of the nimodipine treated control cochleas a mild hydrops was seen during light-microscopic evaluation. Although it was not possible to prove a stimulatory effect of nimodipine on the enzyme Na+/K(+)-ATPase cytochemically, the finding of mild endolymphatic hydrops in nimodipine treated control ears suggests (a history of) increased endolymph production. This hydrops might be responsible for the depression of the CAP in the nimodipine treated ears. SN - 0378-5955 UR - https://www.unboundmedicine.com/medline/citation/7928742/The_effect_of_nimodipine_on_cochlear_potentials_and_Na+/K_+__ATPase_activity_in_normal_and_hydropic_cochleas_of_the_albino_guinea_pig_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-5955(94)90249-6 DB - PRIME DP - Unbound Medicine ER -