Tags

Type your tag names separated by a space and hit enter

A time-lapse study of interactions between Echinostoma paraensei intramolluscan larval stages and adherent hemocytes from Biomphalaria glabrata and Helix aspersa.
J Parasitol. 1994 Oct; 80(5):719-27.JP

Abstract

In vitro interactions between intramolluscan stages (sporocyst, daughter rediae, and metacercariae) of the trematode parasite Echinostoma paraensei and adherent hemocytes from the gastropods Biomphalaria glabrata (intermediate host) and Helix aspersa (non-host) were visualized by time-lapse videomicroscopy. Hemocytes of either species not exposed to E. paraensei displayed extensive mobility and activity of cellular extensions. Image analysis disclosed no significant change in the total surface area occupied by hemocytes in a selected field of view over 2 hr. Echinostoma paraensei exerted life stage-specific effects on the behavior of B. glabrata hemocytes; the cells moved away from sporocysts and daughter rediae but not encysted metacercariae. In the presence of sporocysts, hemocytes rounded up, whereas hemocytes adjacent to rediae assumed a stringy, beady appearance. Hemocytes close to the parasite were affected more rapidly than more distant cells. In 2 hr, a hemocyte-free "halo" formed around the parasite larvae, significantly reducing the hemocyte-occupied surface area (to 43% by sporocysts and to 70% by rediae). The changes induced by sporocysts and rediae are similar to those noted in both in vivo and in vitro studies of the B. glabrata-E. paraensei model system and are interpreted as manifestations of parasite-mediated interference with host hemocyte function. Helix aspersa (non-host) hemocytes were not affected, suggesting that E. paraensei-mediated effects on hemocytes exhibit a degree of specificity.

Authors+Show Affiliations

Biology Department, University of New Mexico, Albuquerque 87131.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7931907

Citation

Adema, C M., et al. "A Time-lapse Study of Interactions Between Echinostoma Paraensei Intramolluscan Larval Stages and Adherent Hemocytes From Biomphalaria Glabrata and Helix Aspersa." The Journal of Parasitology, vol. 80, no. 5, 1994, pp. 719-27.
Adema CM, Arguello DF, Stricker SA, et al. A time-lapse study of interactions between Echinostoma paraensei intramolluscan larval stages and adherent hemocytes from Biomphalaria glabrata and Helix aspersa. J Parasitol. 1994;80(5):719-27.
Adema, C. M., Arguello, D. F., Stricker, S. A., & Loker, E. S. (1994). A time-lapse study of interactions between Echinostoma paraensei intramolluscan larval stages and adherent hemocytes from Biomphalaria glabrata and Helix aspersa. The Journal of Parasitology, 80(5), 719-27.
Adema CM, et al. A Time-lapse Study of Interactions Between Echinostoma Paraensei Intramolluscan Larval Stages and Adherent Hemocytes From Biomphalaria Glabrata and Helix Aspersa. J Parasitol. 1994;80(5):719-27. PubMed PMID: 7931907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A time-lapse study of interactions between Echinostoma paraensei intramolluscan larval stages and adherent hemocytes from Biomphalaria glabrata and Helix aspersa. AU - Adema,C M, AU - Arguello,D F,2nd AU - Stricker,S A, AU - Loker,E S, PY - 1994/10/1/pubmed PY - 2001/3/28/medline PY - 1994/10/1/entrez SP - 719 EP - 27 JF - The Journal of parasitology JO - J Parasitol VL - 80 IS - 5 N2 - In vitro interactions between intramolluscan stages (sporocyst, daughter rediae, and metacercariae) of the trematode parasite Echinostoma paraensei and adherent hemocytes from the gastropods Biomphalaria glabrata (intermediate host) and Helix aspersa (non-host) were visualized by time-lapse videomicroscopy. Hemocytes of either species not exposed to E. paraensei displayed extensive mobility and activity of cellular extensions. Image analysis disclosed no significant change in the total surface area occupied by hemocytes in a selected field of view over 2 hr. Echinostoma paraensei exerted life stage-specific effects on the behavior of B. glabrata hemocytes; the cells moved away from sporocysts and daughter rediae but not encysted metacercariae. In the presence of sporocysts, hemocytes rounded up, whereas hemocytes adjacent to rediae assumed a stringy, beady appearance. Hemocytes close to the parasite were affected more rapidly than more distant cells. In 2 hr, a hemocyte-free "halo" formed around the parasite larvae, significantly reducing the hemocyte-occupied surface area (to 43% by sporocysts and to 70% by rediae). The changes induced by sporocysts and rediae are similar to those noted in both in vivo and in vitro studies of the B. glabrata-E. paraensei model system and are interpreted as manifestations of parasite-mediated interference with host hemocyte function. Helix aspersa (non-host) hemocytes were not affected, suggesting that E. paraensei-mediated effects on hemocytes exhibit a degree of specificity. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/7931907/A_time_lapse_study_of_interactions_between_Echinostoma_paraensei_intramolluscan_larval_stages_and_adherent_hemocytes_from_Biomphalaria_glabrata_and_Helix_aspersa_ DB - PRIME DP - Unbound Medicine ER -