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PCR for the diagnosis of enteroviral meningitis.
Scand J Infect Dis. 1994; 26(3):249-54.SJ

Abstract

A 2-step 'semi-nested' enterovirus PCR was developed and applied to CSF and serum specimens from 27 consecutive patients with aseptic meningitis. CSF and sera from 8 patients with non-enteroviral diagnoses were included as negative clinical controls. Enterovirus RNA was detected in CSF by PCR in 15 of the patients with aseptic meningitis, compared with 6 by virus culture. Acute-phase sera proved positive for enterovirus RNA in 11 patients, thus increasing the number of PCR-positive patients to 18. Convalescent-phase sera were all negative by PCR. The correlation of a positive or negative PCR result in CSF and/or serum versus combined conventional virology (serology and isolation from 1-3 sites, i.e. CSF, stool and throat) was 78%. All negative controls were negative by PCR. PCR is a reliable and sensitive diagnostic tool for the detection of enteroviral infections. Both CSF and acute-phase serum should be considered for testing.

Authors+Show Affiliations

Department of Infectious Diseases, University of Lund, Malmö General Hospital, Sweden.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7939423

Citation

Thorén, A, and A Widell. "PCR for the Diagnosis of Enteroviral Meningitis." Scandinavian Journal of Infectious Diseases, vol. 26, no. 3, 1994, pp. 249-54.
Thorén A, Widell A. PCR for the diagnosis of enteroviral meningitis. Scand J Infect Dis. 1994;26(3):249-54.
Thorén, A., & Widell, A. (1994). PCR for the diagnosis of enteroviral meningitis. Scandinavian Journal of Infectious Diseases, 26(3), 249-54.
Thorén A, Widell A. PCR for the Diagnosis of Enteroviral Meningitis. Scand J Infect Dis. 1994;26(3):249-54. PubMed PMID: 7939423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PCR for the diagnosis of enteroviral meningitis. AU - Thorén,A, AU - Widell,A, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 249 EP - 54 JF - Scandinavian journal of infectious diseases JO - Scand J Infect Dis VL - 26 IS - 3 N2 - A 2-step 'semi-nested' enterovirus PCR was developed and applied to CSF and serum specimens from 27 consecutive patients with aseptic meningitis. CSF and sera from 8 patients with non-enteroviral diagnoses were included as negative clinical controls. Enterovirus RNA was detected in CSF by PCR in 15 of the patients with aseptic meningitis, compared with 6 by virus culture. Acute-phase sera proved positive for enterovirus RNA in 11 patients, thus increasing the number of PCR-positive patients to 18. Convalescent-phase sera were all negative by PCR. The correlation of a positive or negative PCR result in CSF and/or serum versus combined conventional virology (serology and isolation from 1-3 sites, i.e. CSF, stool and throat) was 78%. All negative controls were negative by PCR. PCR is a reliable and sensitive diagnostic tool for the detection of enteroviral infections. Both CSF and acute-phase serum should be considered for testing. SN - 0036-5548 UR - https://www.unboundmedicine.com/medline/citation/7939423/PCR_for_the_diagnosis_of_enteroviral_meningitis_ L2 - https://www.tandfonline.com/doi/full/10.3109/00365549409011792 DB - PRIME DP - Unbound Medicine ER -