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Characterization of monoclonal antibodies against Naja naja oxiana neurotoxin I.
Biochem J. 1994 Oct 01; 303 (Pt 1):163-70.BJ

Abstract

Seven monoclonal antibodies (mAbs) were developed against neurotoxin I (NT-1), a protein from central Asian cobra (Naja naja oxiana) venom which binds specifically to nicotinic acetylcholine receptor (AchR). All of the mAbs cross-reacted with another long-chain post-synaptic neurotoxin, Bungarus multicinctus alpha-bungarotoxin (alpha-BT), but not Naja naja kaouthia alpha-cobratoxin, in an enzyme-linked immunosorbent assay (e.l.i.s.a.). Short-chain post-synaptic neurotoxins like Naja naja atra cobrotoxin, Laticauda semifasciata erabutoxin b, or N. n. oxiana neurotoxin II did not cross-react with the NT-1 mAbs, but an antigen(s) found in Dendroaspis polylepis, Acanthophis antarcticus and Pseudechis australis venoms was immunoreactive. The e.l.i.s.a. readings for dithiothreitol-reduced NT-1 and NT-1 mAbs ranged from 13 to 27% of those for native toxin but reduced alpha-BT was not immunoreactive. Synthetic NT-1 peptides were used in epitope-mapping studies and two, non-contiguous regions (Cys15-Tyr23 and Lys25-Gly33 or Pro17-Lys25 and Asp29-Lys37) were recognized by the NT-1 mAbs. The NT-1 mAbs individually inhibited 31-71% of alpha-BT binding to AchR in vitro and afforded a slight protective effect in vivo with a toxin: antibody mole ratio of 1:1.5. This report is the first to describe mAbs which recognize and protect against a heterologous, long-chain, post-synaptic neurotoxin from snake venom.

Authors+Show Affiliations

Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7945236

Citation

Stiles, B G., et al. "Characterization of Monoclonal Antibodies Against Naja Naja Oxiana Neurotoxin I." The Biochemical Journal, vol. 303 (Pt 1), 1994, pp. 163-70.
Stiles BG, Sexton FW, Guest SB, et al. Characterization of monoclonal antibodies against Naja naja oxiana neurotoxin I. Biochem J. 1994;303 (Pt 1):163-70.
Stiles, B. G., Sexton, F. W., Guest, S. B., Olson, M. A., & Hack, D. C. (1994). Characterization of monoclonal antibodies against Naja naja oxiana neurotoxin I. The Biochemical Journal, 303 (Pt 1), 163-70.
Stiles BG, et al. Characterization of Monoclonal Antibodies Against Naja Naja Oxiana Neurotoxin I. Biochem J. 1994 Oct 1;303 (Pt 1):163-70. PubMed PMID: 7945236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of monoclonal antibodies against Naja naja oxiana neurotoxin I. AU - Stiles,B G, AU - Sexton,F W, AU - Guest,S B, AU - Olson,M A, AU - Hack,D C, PY - 1994/10/1/pubmed PY - 1994/10/1/medline PY - 1994/10/1/entrez SP - 163 EP - 70 JF - The Biochemical journal JO - Biochem. J. VL - 303 (Pt 1) N2 - Seven monoclonal antibodies (mAbs) were developed against neurotoxin I (NT-1), a protein from central Asian cobra (Naja naja oxiana) venom which binds specifically to nicotinic acetylcholine receptor (AchR). All of the mAbs cross-reacted with another long-chain post-synaptic neurotoxin, Bungarus multicinctus alpha-bungarotoxin (alpha-BT), but not Naja naja kaouthia alpha-cobratoxin, in an enzyme-linked immunosorbent assay (e.l.i.s.a.). Short-chain post-synaptic neurotoxins like Naja naja atra cobrotoxin, Laticauda semifasciata erabutoxin b, or N. n. oxiana neurotoxin II did not cross-react with the NT-1 mAbs, but an antigen(s) found in Dendroaspis polylepis, Acanthophis antarcticus and Pseudechis australis venoms was immunoreactive. The e.l.i.s.a. readings for dithiothreitol-reduced NT-1 and NT-1 mAbs ranged from 13 to 27% of those for native toxin but reduced alpha-BT was not immunoreactive. Synthetic NT-1 peptides were used in epitope-mapping studies and two, non-contiguous regions (Cys15-Tyr23 and Lys25-Gly33 or Pro17-Lys25 and Asp29-Lys37) were recognized by the NT-1 mAbs. The NT-1 mAbs individually inhibited 31-71% of alpha-BT binding to AchR in vitro and afforded a slight protective effect in vivo with a toxin: antibody mole ratio of 1:1.5. This report is the first to describe mAbs which recognize and protect against a heterologous, long-chain, post-synaptic neurotoxin from snake venom. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/7945236/Characterization_of_monoclonal_antibodies_against_Naja_naja_oxiana_neurotoxin_I_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj3030163 DB - PRIME DP - Unbound Medicine ER -