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Cloning, analysis and expression of an rpoS homologue gene from Pseudomonas aeruginosa PAO1.
Gene. 1994 Dec 02; 150(1):81-5.GENE

Abstract

A homologue of the rpoS gene of Escherichia coli was cloned from Pseudomonas aeruginosa PAO1 by hybridization with an oligodeoxyribonucleotide probe designed from an amino-acid stretch conserved among the principal sigma factors of eubacteria. Two open reading frames, the pcm gene and the orf-297 of unknown function, were found in the upstream region of rpoS, and in the same order as in E. coli. The rpoS gene of P. aeruginosa was expressed in E. coli and complemented the catalase deficiency of the rpoS mutant of E. coli. The RpoS protein of P. aeruginosa was identified by Western blot analysis in both P. aeruginosa (Pa) and the transformed E. coli. Levels of RpoS of Pa increased drastically at the onset of the stationary growth phase.

Authors+Show Affiliations

Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7959068

Citation

Tanaka, K, and H Takahashi. "Cloning, Analysis and Expression of an rpoS Homologue Gene From Pseudomonas Aeruginosa PAO1." Gene, vol. 150, no. 1, 1994, pp. 81-5.
Tanaka K, Takahashi H. Cloning, analysis and expression of an rpoS homologue gene from Pseudomonas aeruginosa PAO1. Gene. 1994;150(1):81-5.
Tanaka, K., & Takahashi, H. (1994). Cloning, analysis and expression of an rpoS homologue gene from Pseudomonas aeruginosa PAO1. Gene, 150(1), 81-5.
Tanaka K, Takahashi H. Cloning, Analysis and Expression of an rpoS Homologue Gene From Pseudomonas Aeruginosa PAO1. Gene. 1994 Dec 2;150(1):81-5. PubMed PMID: 7959068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning, analysis and expression of an rpoS homologue gene from Pseudomonas aeruginosa PAO1. AU - Tanaka,K, AU - Takahashi,H, PY - 1994/12/2/pubmed PY - 1994/12/2/medline PY - 1994/12/2/entrez SP - 81 EP - 5 JF - Gene JO - Gene VL - 150 IS - 1 N2 - A homologue of the rpoS gene of Escherichia coli was cloned from Pseudomonas aeruginosa PAO1 by hybridization with an oligodeoxyribonucleotide probe designed from an amino-acid stretch conserved among the principal sigma factors of eubacteria. Two open reading frames, the pcm gene and the orf-297 of unknown function, were found in the upstream region of rpoS, and in the same order as in E. coli. The rpoS gene of P. aeruginosa was expressed in E. coli and complemented the catalase deficiency of the rpoS mutant of E. coli. The RpoS protein of P. aeruginosa was identified by Western blot analysis in both P. aeruginosa (Pa) and the transformed E. coli. Levels of RpoS of Pa increased drastically at the onset of the stationary growth phase. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/7959068/Cloning_analysis_and_expression_of_an_rpoS_homologue_gene_from_Pseudomonas_aeruginosa_PAO1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(94)90862-1 DB - PRIME DP - Unbound Medicine ER -