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Mutational analysis of the transcriptional activator VirG of Agrobacterium tumefaciens.
J Bacteriol. 1994 Nov; 176(21):6418-26.JB

Abstract

To find VirG proteins with altered properties, the virG gene was mutagenized. Random chemical mutagenesis of single-stranded DNA containing the Agrobacterium tumefaciens virG gene led with high frequency to the inactivation of the gene. Sequence analysis showed that 29% of the mutants contained a virG gene with one single-base-pair substitution somewhere in the open reading frame. Thirty-nine different mutations that rendered the VirG protein inactive were mapped. Besides these inactive mutants, two mutants in which the vir genes were active even in the absence of acetosyringone were found on indicator plates. A VirG protein with an N54D substitution turned out to be able to induce a virB-lacZ reporter gene to a high level even in the absence of the inducer acetosyringone. A VirG protein with an I77V substitution exhibited almost no induction in the absence of acetosyringone but showed a maximum induction level already at low concentrations of acetosyringone.

Authors+Show Affiliations

Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7961391

Citation

Scheeren-Groot, E P., et al. "Mutational Analysis of the Transcriptional Activator VirG of Agrobacterium Tumefaciens." Journal of Bacteriology, vol. 176, no. 21, 1994, pp. 6418-26.
Scheeren-Groot EP, Rodenburg KW, den Dulk-Ras A, et al. Mutational analysis of the transcriptional activator VirG of Agrobacterium tumefaciens. J Bacteriol. 1994;176(21):6418-26.
Scheeren-Groot, E. P., Rodenburg, K. W., den Dulk-Ras, A., Turk, S. C., & Hooykaas, P. J. (1994). Mutational analysis of the transcriptional activator VirG of Agrobacterium tumefaciens. Journal of Bacteriology, 176(21), 6418-26.
Scheeren-Groot EP, et al. Mutational Analysis of the Transcriptional Activator VirG of Agrobacterium Tumefaciens. J Bacteriol. 1994;176(21):6418-26. PubMed PMID: 7961391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutational analysis of the transcriptional activator VirG of Agrobacterium tumefaciens. AU - Scheeren-Groot,E P, AU - Rodenburg,K W, AU - den Dulk-Ras,A, AU - Turk,S C, AU - Hooykaas,P J, PY - 1994/11/1/pubmed PY - 1994/11/1/medline PY - 1994/11/1/entrez SP - 6418 EP - 26 JF - Journal of bacteriology JO - J. Bacteriol. VL - 176 IS - 21 N2 - To find VirG proteins with altered properties, the virG gene was mutagenized. Random chemical mutagenesis of single-stranded DNA containing the Agrobacterium tumefaciens virG gene led with high frequency to the inactivation of the gene. Sequence analysis showed that 29% of the mutants contained a virG gene with one single-base-pair substitution somewhere in the open reading frame. Thirty-nine different mutations that rendered the VirG protein inactive were mapped. Besides these inactive mutants, two mutants in which the vir genes were active even in the absence of acetosyringone were found on indicator plates. A VirG protein with an N54D substitution turned out to be able to induce a virB-lacZ reporter gene to a high level even in the absence of the inducer acetosyringone. A VirG protein with an I77V substitution exhibited almost no induction in the absence of acetosyringone but showed a maximum induction level already at low concentrations of acetosyringone. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/7961391/Mutational_analysis_of_the_transcriptional_activator_VirG_of_Agrobacterium_tumefaciens_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=7961391 DB - PRIME DP - Unbound Medicine ER -