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Superoxide and peroxynitrite inactivate aconitases, but nitric oxide does not.
J Biol Chem. 1994 Nov 25; 269(47):29405-8.JB

Abstract

The Escherichia coli and recombinant human cytosolic aconitases are inactivated by O2-., with a rate constant of approximately 3 x 10(7) M-1 s-1; the corresponding value for the porcine mitochondrial aconitase is approximately 0.8 x 10(7) M-1 s-1. Nitric oxide, which is reported to inactivate aconitase, did not do so at a perceptible rate, while incubation with peroxynitrite led to a rapid loss of aconitase activity. We propose that the reported inactivation of aconitase by nitric oxide in vivo is actually mediated through peroxynitrite, the product of the reaction between O2-. and NO..

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Authors+Show Affiliations

Hausladen A
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Fridovich I
No affiliation info available

MeSH

Aconitate HydrataseAnimalsEscherichia coliHumansNitratesNitric OxideSuperoxidesSwine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7961919

Citation

Hausladen, A, and I Fridovich. "Superoxide and Peroxynitrite Inactivate Aconitases, but Nitric Oxide Does Not." The Journal of Biological Chemistry, vol. 269, no. 47, 1994, pp. 29405-8.
Hausladen A, Fridovich I. Superoxide and peroxynitrite inactivate aconitases, but nitric oxide does not. J Biol Chem. 1994;269(47):29405-8.
Hausladen, A., & Fridovich, I. (1994). Superoxide and peroxynitrite inactivate aconitases, but nitric oxide does not. The Journal of Biological Chemistry, 269(47), 29405-8.
Hausladen A, Fridovich I. Superoxide and Peroxynitrite Inactivate Aconitases, but Nitric Oxide Does Not. J Biol Chem. 1994 Nov 25;269(47):29405-8. PubMed PMID: 7961919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Superoxide and peroxynitrite inactivate aconitases, but nitric oxide does not. AU - Hausladen,A, AU - Fridovich,I, PY - 1994/11/25/pubmed PY - 1994/11/25/medline PY - 1994/11/25/entrez SP - 29405 EP - 8 JF - The Journal of biological chemistry JO - J Biol Chem VL - 269 IS - 47 N2 - The Escherichia coli and recombinant human cytosolic aconitases are inactivated by O2-., with a rate constant of approximately 3 x 10(7) M-1 s-1; the corresponding value for the porcine mitochondrial aconitase is approximately 0.8 x 10(7) M-1 s-1. Nitric oxide, which is reported to inactivate aconitase, did not do so at a perceptible rate, while incubation with peroxynitrite led to a rapid loss of aconitase activity. We propose that the reported inactivation of aconitase by nitric oxide in vivo is actually mediated through peroxynitrite, the product of the reaction between O2-. and NO.. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/7961919/Superoxide_and_peroxynitrite_inactivate_aconitases_but_nitric_oxide_does_not_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(18)43893-8 DB - PRIME DP - Unbound Medicine ER -