Tags

Type your tag names separated by a space and hit enter

Inhibition of presynaptic catecholamine synthesis with alpha-methyl-para-tyrosine attenuates nocturnal melatonin secretion in humans.
J Clin Endocrinol Metab 1994; 79(4):1110-4JC

Abstract

The catecholamine dopamine (D) is involved in the regulation of LH and PRL secretion, whereas a dysregulated noradrenergic system may contribute significantly to symptoms encountered in affective disorders. This explains the attraction of using alpha-methyl-para-tyrosine (AMPT) in neuroendocrine and psychiatric research, as it inhibits both neurotransmitters. PRL has been used as a marker of the effectiveness of AMPT in blocking D function, but no good marker for the effectiveness of AMPT in blocking norepinephrine (NE) is available. The purpose of this study was to determine whether melatonin (M) might serve as such a marker, as its production and secretion are regulated by NE. Seven subjects were given either AMPT or promethazine, which does not alter M secretion, in a randomized, double blind fashion, and 24-h M secretion was studied. Two-way analysis of variance revealed a significant difference in M secretion (F = 13.2; df = 17,102; P = 0.0013), with the following time points being different: 22, 23, 24, 1, 2, 3, 4, 5, and 6 h. Also, 24-h urinary 6-sulfatoxymelatonin excretion correlated highly with 24-h M secretion, expressed as the area under the curve in the AMPT experiment (r = 0.93; P = 0.002), which indicates that AMPT does not alter the metabolism of M. These results demonstrate for the first time that AMPT significantly attenuates nocturnal M secretion. It is concluded that M is a good marker for characterizing the effectiveness of AMPT in inhibiting sympathetic NE activity.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, Minnesota 55905.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7962283

Citation

Zimmermann, R C., et al. "Inhibition of Presynaptic Catecholamine Synthesis With Alpha-methyl-para-tyrosine Attenuates Nocturnal Melatonin Secretion in Humans." The Journal of Clinical Endocrinology and Metabolism, vol. 79, no. 4, 1994, pp. 1110-4.
Zimmermann RC, Krahn L, Klee G, et al. Inhibition of presynaptic catecholamine synthesis with alpha-methyl-para-tyrosine attenuates nocturnal melatonin secretion in humans. J Clin Endocrinol Metab. 1994;79(4):1110-4.
Zimmermann, R. C., Krahn, L., Klee, G., Delgado, P., Ory, S. J., & Lin, S. C. (1994). Inhibition of presynaptic catecholamine synthesis with alpha-methyl-para-tyrosine attenuates nocturnal melatonin secretion in humans. The Journal of Clinical Endocrinology and Metabolism, 79(4), pp. 1110-4.
Zimmermann RC, et al. Inhibition of Presynaptic Catecholamine Synthesis With Alpha-methyl-para-tyrosine Attenuates Nocturnal Melatonin Secretion in Humans. J Clin Endocrinol Metab. 1994;79(4):1110-4. PubMed PMID: 7962283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of presynaptic catecholamine synthesis with alpha-methyl-para-tyrosine attenuates nocturnal melatonin secretion in humans. AU - Zimmermann,R C, AU - Krahn,L, AU - Klee,G, AU - Delgado,P, AU - Ory,S J, AU - Lin,S C, PY - 1994/10/1/pubmed PY - 1994/10/1/medline PY - 1994/10/1/entrez SP - 1110 EP - 4 JF - The Journal of clinical endocrinology and metabolism JO - J. Clin. Endocrinol. Metab. VL - 79 IS - 4 N2 - The catecholamine dopamine (D) is involved in the regulation of LH and PRL secretion, whereas a dysregulated noradrenergic system may contribute significantly to symptoms encountered in affective disorders. This explains the attraction of using alpha-methyl-para-tyrosine (AMPT) in neuroendocrine and psychiatric research, as it inhibits both neurotransmitters. PRL has been used as a marker of the effectiveness of AMPT in blocking D function, but no good marker for the effectiveness of AMPT in blocking norepinephrine (NE) is available. The purpose of this study was to determine whether melatonin (M) might serve as such a marker, as its production and secretion are regulated by NE. Seven subjects were given either AMPT or promethazine, which does not alter M secretion, in a randomized, double blind fashion, and 24-h M secretion was studied. Two-way analysis of variance revealed a significant difference in M secretion (F = 13.2; df = 17,102; P = 0.0013), with the following time points being different: 22, 23, 24, 1, 2, 3, 4, 5, and 6 h. Also, 24-h urinary 6-sulfatoxymelatonin excretion correlated highly with 24-h M secretion, expressed as the area under the curve in the AMPT experiment (r = 0.93; P = 0.002), which indicates that AMPT does not alter the metabolism of M. These results demonstrate for the first time that AMPT significantly attenuates nocturnal M secretion. It is concluded that M is a good marker for characterizing the effectiveness of AMPT in inhibiting sympathetic NE activity. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/7962283/Inhibition_of_presynaptic_catecholamine_synthesis_with_alpha_methyl_para_tyrosine_attenuates_nocturnal_melatonin_secretion_in_humans_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.79.4.7962283 DB - PRIME DP - Unbound Medicine ER -