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Electrophysiological verification of the presence of D1 and D2 dopamine receptors within the ventral pallidum.
Synapse. 1994 Jul; 17(3):160-6.S

Abstract

The ventral pallidum is a basal forebrain region recently shown to receive dopaminergic projections from the midbrain. Binding sites for the D1 and D2 dopamine receptor families have been identified within the ventral pallidum, yet the consequences of activating these receptors have not been studied. Thus, to characterize the physiological pharmacology of D1 and D2 receptor subtypes for the ventral pallidum, extracellular single-neuron recording and microiontophoretic techniques were used in chloral hydrate-anesthetized rats. Half of the 93 ventral pallidal neurons tested were sensitive to iontophoresis of dopamine (DA), and both rate increases and decreases were observed. Co-iontophoresis of either the D1 antagonist SCH23390, or the D2 antagonist sulpiride, generally attenuated the DA-induced rate changes. Like DA, about half of the ventral pallidal neurons tested were sensitive to the D1 agonist, SKF38393. Yet in contrast to DA, rate suppression was observed almost exclusively, and the magnitude of this decrease was greater than that produced by DA. SKF38393-induced suppressions were antagonized by SCH23390, but not by sulpiride, demonstrating the specificity of the D1 agonist. Most of the neurons tested were not affected by quinpirole, but when responsive to the D2 agonist, rate increases were observed most often. The increases were antagonized by the D2 antagonist sulpiride, but not SCH23390, demonstrating that this response resulted from an activation of D2 receptors. These results support binding studies demonstrating that both D1 and D2 receptors are present in the ventral pallidum, and reveal that the independent activation of each of these is sufficient to alter neuronal activity.

Authors+Show Affiliations

Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois 60153.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7974198

Citation

Napier, T C., and R J. Maslowski-Cobuzzi. "Electrophysiological Verification of the Presence of D1 and D2 Dopamine Receptors Within the Ventral Pallidum." Synapse (New York, N.Y.), vol. 17, no. 3, 1994, pp. 160-6.
Napier TC, Maslowski-Cobuzzi RJ. Electrophysiological verification of the presence of D1 and D2 dopamine receptors within the ventral pallidum. Synapse. 1994;17(3):160-6.
Napier, T. C., & Maslowski-Cobuzzi, R. J. (1994). Electrophysiological verification of the presence of D1 and D2 dopamine receptors within the ventral pallidum. Synapse (New York, N.Y.), 17(3), 160-6.
Napier TC, Maslowski-Cobuzzi RJ. Electrophysiological Verification of the Presence of D1 and D2 Dopamine Receptors Within the Ventral Pallidum. Synapse. 1994;17(3):160-6. PubMed PMID: 7974198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrophysiological verification of the presence of D1 and D2 dopamine receptors within the ventral pallidum. AU - Napier,T C, AU - Maslowski-Cobuzzi,R J, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 160 EP - 6 JF - Synapse (New York, N.Y.) JO - Synapse VL - 17 IS - 3 N2 - The ventral pallidum is a basal forebrain region recently shown to receive dopaminergic projections from the midbrain. Binding sites for the D1 and D2 dopamine receptor families have been identified within the ventral pallidum, yet the consequences of activating these receptors have not been studied. Thus, to characterize the physiological pharmacology of D1 and D2 receptor subtypes for the ventral pallidum, extracellular single-neuron recording and microiontophoretic techniques were used in chloral hydrate-anesthetized rats. Half of the 93 ventral pallidal neurons tested were sensitive to iontophoresis of dopamine (DA), and both rate increases and decreases were observed. Co-iontophoresis of either the D1 antagonist SCH23390, or the D2 antagonist sulpiride, generally attenuated the DA-induced rate changes. Like DA, about half of the ventral pallidal neurons tested were sensitive to the D1 agonist, SKF38393. Yet in contrast to DA, rate suppression was observed almost exclusively, and the magnitude of this decrease was greater than that produced by DA. SKF38393-induced suppressions were antagonized by SCH23390, but not by sulpiride, demonstrating the specificity of the D1 agonist. Most of the neurons tested were not affected by quinpirole, but when responsive to the D2 agonist, rate increases were observed most often. The increases were antagonized by the D2 antagonist sulpiride, but not SCH23390, demonstrating that this response resulted from an activation of D2 receptors. These results support binding studies demonstrating that both D1 and D2 receptors are present in the ventral pallidum, and reveal that the independent activation of each of these is sufficient to alter neuronal activity. SN - 0887-4476 UR - https://www.unboundmedicine.com/medline/citation/7974198/Electrophysiological_verification_of_the_presence_of_D1_and_D2_dopamine_receptors_within_the_ventral_pallidum_ L2 - https://doi.org/10.1002/syn.890170304 DB - PRIME DP - Unbound Medicine ER -