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Nitric oxide inhibition causes intrauterine growth retardation and hind-limb disruptions in rats.
Am J Obstet Gynecol. 1994 Nov; 171(5):1243-50.AJ

Abstract

OBJECTIVE

Our purpose was to determine the effects of nitric oxide synthase inhibition on maternal and fetal health in the last third of pregnancy.

STUDY DESIGN

Pregnant rats were treated from gestational day 13 to day 19 or 20 with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester, which was administered in the drinking water ad libitum. Control animals received the inactive enantiomer NG-nitro-D-arginine methyl ester or no treatment. Maternal blood pressure, blood chemistry studies, and placenta and pup size were determined. A separate group of rats received nitroprusside sodium in conjunction with NG-nitro-L-arginine methyl ester.

RESULTS

NG-nitro-L-arginine methyl ester caused a dose-dependent reduction in placenta and pup size. Amniotic fluid levels of cyclic guanosine monophosphate were significantly reduced at 0.1 mg/ml but not at higher doses. Hemorrhagic necrosis of fetal hind limbs occurred only with treatment with NG-nitro-L-arginine methyl ester and was prevented by coadministration of nitroprusside sodium. Maternal blood pressure and blood and urine chemistry studies were unaffected by NG-nitro-L-arginine methyl ester.

CONCLUSION

Chronic reductions of nitric oxide production in the last third of pregnancy result in significant intrauterine growth retardation and hemorrhagic disruptions of hind limbs. Maternal complications were minimal and did not mimic preeclampsia.

Authors+Show Affiliations

Department of Pediatrics, Louisiana State University Medical Center, New Orleans 70112-2822.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7977528

Citation

Diket, A L., et al. "Nitric Oxide Inhibition Causes Intrauterine Growth Retardation and Hind-limb Disruptions in Rats." American Journal of Obstetrics and Gynecology, vol. 171, no. 5, 1994, pp. 1243-50.
Diket AL, Pierce MR, Munshi UK, et al. Nitric oxide inhibition causes intrauterine growth retardation and hind-limb disruptions in rats. Am J Obstet Gynecol. 1994;171(5):1243-50.
Diket, A. L., Pierce, M. R., Munshi, U. K., Voelker, C. A., Eloby-Childress, S., Greenberg, S. S., Zhang, X. J., Clark, D. A., & Miller, M. J. (1994). Nitric oxide inhibition causes intrauterine growth retardation and hind-limb disruptions in rats. American Journal of Obstetrics and Gynecology, 171(5), 1243-50.
Diket AL, et al. Nitric Oxide Inhibition Causes Intrauterine Growth Retardation and Hind-limb Disruptions in Rats. Am J Obstet Gynecol. 1994;171(5):1243-50. PubMed PMID: 7977528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide inhibition causes intrauterine growth retardation and hind-limb disruptions in rats. AU - Diket,A L, AU - Pierce,M R, AU - Munshi,U K, AU - Voelker,C A, AU - Eloby-Childress,S, AU - Greenberg,S S, AU - Zhang,X J, AU - Clark,D A, AU - Miller,M J, PY - 1994/11/1/pubmed PY - 1994/11/1/medline PY - 1994/11/1/entrez SP - 1243 EP - 50 JF - American journal of obstetrics and gynecology JO - Am J Obstet Gynecol VL - 171 IS - 5 N2 - OBJECTIVE: Our purpose was to determine the effects of nitric oxide synthase inhibition on maternal and fetal health in the last third of pregnancy. STUDY DESIGN: Pregnant rats were treated from gestational day 13 to day 19 or 20 with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester, which was administered in the drinking water ad libitum. Control animals received the inactive enantiomer NG-nitro-D-arginine methyl ester or no treatment. Maternal blood pressure, blood chemistry studies, and placenta and pup size were determined. A separate group of rats received nitroprusside sodium in conjunction with NG-nitro-L-arginine methyl ester. RESULTS: NG-nitro-L-arginine methyl ester caused a dose-dependent reduction in placenta and pup size. Amniotic fluid levels of cyclic guanosine monophosphate were significantly reduced at 0.1 mg/ml but not at higher doses. Hemorrhagic necrosis of fetal hind limbs occurred only with treatment with NG-nitro-L-arginine methyl ester and was prevented by coadministration of nitroprusside sodium. Maternal blood pressure and blood and urine chemistry studies were unaffected by NG-nitro-L-arginine methyl ester. CONCLUSION: Chronic reductions of nitric oxide production in the last third of pregnancy result in significant intrauterine growth retardation and hemorrhagic disruptions of hind limbs. Maternal complications were minimal and did not mimic preeclampsia. SN - 0002-9378 UR - https://www.unboundmedicine.com/medline/citation/7977528/Nitric_oxide_inhibition_causes_intrauterine_growth_retardation_and_hind_limb_disruptions_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0002-9378(94)90141-4 DB - PRIME DP - Unbound Medicine ER -