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Characterization of two monoclonal antibodies directed against serovar A Haemophilus paragallinarum.
Avian Dis 1994 Apr-Jun; 38(2):361-5AD

Abstract

Two monoclonal antibodies (MAbs) raised against a serovar A Haemophilus paragallinarum were evaluated for their ability to react with 11 reference strains that represented all the recognized serovars and with 27 field isolates of Page serovar A collected from around the world. The MAbs were used in a hemagglutination-inhibition assay. Both MAbs recognized type strains of Page serovar A and Kume serovars A-1 and A-2 but not the type strains of Kume serovars A-3 and A-4. Neither MAb recognized the type strains of Page serovars B and C or Kume serovars B-1, C-1, C-2, C-3, or C-4. When evaluated with the 27 Page serovar A field isolates, both MAbs recognized only 10 isolates. All of the recognized isolates belonged to Kume serovars A-1 (nine isolates) or A-2 (one isolate). All of the field isolates that were not recognized by one or the other of the MAbs either were Kume serovar A-4 (seven isolates) or could not be placed in an existing Kume A serovar (10 isolates). The results indicate that the epitope recognized by these MAbs is present only in strains of H. paragallinarum that belong to Kume serovars A-1 and A-2.

Authors+Show Affiliations

Queensland Department of Primary Industries, Animal Research Institute, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7980289

Citation

Blackall, P J., et al. "Characterization of Two Monoclonal Antibodies Directed Against Serovar a Haemophilus Paragallinarum." Avian Diseases, vol. 38, no. 2, 1994, pp. 361-5.
Blackall PJ, Zheng YZ, Takagi M, et al. Characterization of two monoclonal antibodies directed against serovar A Haemophilus paragallinarum. Avian Dis. 1994;38(2):361-5.
Blackall, P. J., Zheng, Y. Z., Takagi, M., Terzolo, H. R., Sandoval, V. E., & Silva, E. N. (1994). Characterization of two monoclonal antibodies directed against serovar A Haemophilus paragallinarum. Avian Diseases, 38(2), pp. 361-5.
Blackall PJ, et al. Characterization of Two Monoclonal Antibodies Directed Against Serovar a Haemophilus Paragallinarum. Avian Dis. 1994;38(2):361-5. PubMed PMID: 7980289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of two monoclonal antibodies directed against serovar A Haemophilus paragallinarum. AU - Blackall,P J, AU - Zheng,Y Z, AU - Takagi,M, AU - Terzolo,H R, AU - Sandoval,V E, AU - Silva,E N, PY - 1994/4/1/pubmed PY - 1994/4/1/medline PY - 1994/4/1/entrez SP - 361 EP - 5 JF - Avian diseases JO - Avian Dis. VL - 38 IS - 2 N2 - Two monoclonal antibodies (MAbs) raised against a serovar A Haemophilus paragallinarum were evaluated for their ability to react with 11 reference strains that represented all the recognized serovars and with 27 field isolates of Page serovar A collected from around the world. The MAbs were used in a hemagglutination-inhibition assay. Both MAbs recognized type strains of Page serovar A and Kume serovars A-1 and A-2 but not the type strains of Kume serovars A-3 and A-4. Neither MAb recognized the type strains of Page serovars B and C or Kume serovars B-1, C-1, C-2, C-3, or C-4. When evaluated with the 27 Page serovar A field isolates, both MAbs recognized only 10 isolates. All of the recognized isolates belonged to Kume serovars A-1 (nine isolates) or A-2 (one isolate). All of the field isolates that were not recognized by one or the other of the MAbs either were Kume serovar A-4 (seven isolates) or could not be placed in an existing Kume A serovar (10 isolates). The results indicate that the epitope recognized by these MAbs is present only in strains of H. paragallinarum that belong to Kume serovars A-1 and A-2. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/7980289/Characterization_of_two_monoclonal_antibodies_directed_against_serovar_A_Haemophilus_paragallinarum_ DB - PRIME DP - Unbound Medicine ER -