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Role of rpoS in the regulation of Salmonella plasmid virulence (spv) genes.
FEMS Microbiol Lett. 1994 Oct 15; 123(1-2):125-30.FM

Abstract

Salmonella plasmid virulence (spv) genes are organized into two transcriptional units: one formed by the spvR gene and the other by the spvA, spvB, spvC and spvD genes. Transcription of both units is activated by SpvR, a regulatory protein of the LysR family. The effect of RpoS, a stationary phase-associated sigma factor, on the expression of spv genes was studied using lacZ transcriptional fusions to spvR and spvA in wild-type and rpoS Escherichia coli backgrounds. Mutant and wild-type SpvR proteins were expressed in trans from a multicopy plasmid. The results show that the combined action of rpoS and spvR is necessary for transcription of spvA and that this combination also enhances transcription of spvR. Interestingly, spvR can also be transcribed in an alternative manner, i.e. in the absence of rpoS or spvR or both. The possible role for SpvR as a repressor of its own transcription is discussed.

Authors+Show Affiliations

Department of Biochemistry, University of Helsinki, Finland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7988879

Citation

Heiskanen, P, et al. "Role of rpoS in the Regulation of Salmonella Plasmid Virulence (spv) Genes." FEMS Microbiology Letters, vol. 123, no. 1-2, 1994, pp. 125-30.
Heiskanen P, Taira S, Rhen M. Role of rpoS in the regulation of Salmonella plasmid virulence (spv) genes. FEMS Microbiol Lett. 1994;123(1-2):125-30.
Heiskanen, P., Taira, S., & Rhen, M. (1994). Role of rpoS in the regulation of Salmonella plasmid virulence (spv) genes. FEMS Microbiology Letters, 123(1-2), 125-30.
Heiskanen P, Taira S, Rhen M. Role of rpoS in the Regulation of Salmonella Plasmid Virulence (spv) Genes. FEMS Microbiol Lett. 1994 Oct 15;123(1-2):125-30. PubMed PMID: 7988879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of rpoS in the regulation of Salmonella plasmid virulence (spv) genes. AU - Heiskanen,P, AU - Taira,S, AU - Rhen,M, PY - 1994/10/15/pubmed PY - 1994/10/15/medline PY - 1994/10/15/entrez SP - 125 EP - 30 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 123 IS - 1-2 N2 - Salmonella plasmid virulence (spv) genes are organized into two transcriptional units: one formed by the spvR gene and the other by the spvA, spvB, spvC and spvD genes. Transcription of both units is activated by SpvR, a regulatory protein of the LysR family. The effect of RpoS, a stationary phase-associated sigma factor, on the expression of spv genes was studied using lacZ transcriptional fusions to spvR and spvA in wild-type and rpoS Escherichia coli backgrounds. Mutant and wild-type SpvR proteins were expressed in trans from a multicopy plasmid. The results show that the combined action of rpoS and spvR is necessary for transcription of spvA and that this combination also enhances transcription of spvR. Interestingly, spvR can also be transcribed in an alternative manner, i.e. in the absence of rpoS or spvR or both. The possible role for SpvR as a repressor of its own transcription is discussed. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/7988879/Role_of_rpoS_in_the_regulation_of_Salmonella_plasmid_virulence__spv__genes_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.1994.tb07211.x DB - PRIME DP - Unbound Medicine ER -