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Analysis of beta-glucans and chitin in a Saccharomyces cerevisiae cell wall mutant using high-performance liquid chromatography.
Yeast. 1994 Aug; 10(8):1083-92.Y

Abstract

We have previously shown that mutations in the yeast KNR4 gene resulted in pleiotropic cell wall defects, including resistance to killer 9 toxin, elevated osmotic sensitivity to SDS and increased resistance to zymolyase, a (1-->3)-beta-glucanase. In this report, we further demonstrated that knr4 mutant cells were more permeable to a chromogenic substrate, X-GAL, suggesting that the mutant cell walls were leakier to certain non-permeable molecules. To determine if these defects resulted from structural changes in the cell walls, we analysed the alkali-insoluble cell wall components using HPLC assays developed for this purpose. Comparative analysis using four isogenic strains from a 'knr4 disrupted' tetrad demonstrated that mutant cell walls contained much less (1-->3)-beta-glucan and (1-->6)-beta-glucan; however, the level of chitin, a minor cell wall component, was found to be five times higher in the mutant strains compared to the wild-type strains. The data suggested that the knr4 mutant cell walls were dramatically weakened, which may explain the pleiotropic cell wall defects.

Authors+Show Affiliations

Hong Z
Chemotherapy and Molecular Genetics, Schering-Plough Research Institute, Kenilworth, New Jersey 07033-0539.
Mann P
No affiliation info available
Shaw KJ
No affiliation info available
Didomenico B
No affiliation info available

MeSH

Cell WallChitinChromatography, High Pressure LiquidFungal ProteinsGalactosidesGlucansIndolesMutationPermeabilitySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription Factorsbeta-Glucans

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7992508

Citation

Hong, Z, et al. "Analysis of Beta-glucans and Chitin in a Saccharomyces Cerevisiae Cell Wall Mutant Using High-performance Liquid Chromatography." Yeast (Chichester, England), vol. 10, no. 8, 1994, pp. 1083-92.
Hong Z, Mann P, Shaw KJ, et al. Analysis of beta-glucans and chitin in a Saccharomyces cerevisiae cell wall mutant using high-performance liquid chromatography. Yeast. 1994;10(8):1083-92.
Hong, Z., Mann, P., Shaw, K. J., & Didomenico, B. (1994). Analysis of beta-glucans and chitin in a Saccharomyces cerevisiae cell wall mutant using high-performance liquid chromatography. Yeast (Chichester, England), 10(8), 1083-92.
Hong Z, et al. Analysis of Beta-glucans and Chitin in a Saccharomyces Cerevisiae Cell Wall Mutant Using High-performance Liquid Chromatography. Yeast. 1994;10(8):1083-92. PubMed PMID: 7992508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of beta-glucans and chitin in a Saccharomyces cerevisiae cell wall mutant using high-performance liquid chromatography. AU - Hong,Z, AU - Mann,P, AU - Shaw,K J, AU - Didomenico,B, PY - 1994/8/1/pubmed PY - 1994/8/1/medline PY - 1994/8/1/entrez SP - 1083 EP - 92 JF - Yeast (Chichester, England) JO - Yeast VL - 10 IS - 8 N2 - We have previously shown that mutations in the yeast KNR4 gene resulted in pleiotropic cell wall defects, including resistance to killer 9 toxin, elevated osmotic sensitivity to SDS and increased resistance to zymolyase, a (1-->3)-beta-glucanase. In this report, we further demonstrated that knr4 mutant cells were more permeable to a chromogenic substrate, X-GAL, suggesting that the mutant cell walls were leakier to certain non-permeable molecules. To determine if these defects resulted from structural changes in the cell walls, we analysed the alkali-insoluble cell wall components using HPLC assays developed for this purpose. Comparative analysis using four isogenic strains from a 'knr4 disrupted' tetrad demonstrated that mutant cell walls contained much less (1-->3)-beta-glucan and (1-->6)-beta-glucan; however, the level of chitin, a minor cell wall component, was found to be five times higher in the mutant strains compared to the wild-type strains. The data suggested that the knr4 mutant cell walls were dramatically weakened, which may explain the pleiotropic cell wall defects. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/7992508/Analysis_of_beta_glucans_and_chitin_in_a_Saccharomyces_cerevisiae_cell_wall_mutant_using_high_performance_liquid_chromatography_ L2 - https://doi.org/10.1002/yea.320100810 DB - PRIME DP - Unbound Medicine ER -