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Development of a live, oral, attenuated vaccine against El Tor cholera.
J Infect Dis. 1994 Dec; 170(6):1518-23.JI

Abstract

Vibrio cholerae El Tor strains from Peru, Bangladesh, and Bahrain were attenuated by deletion of a genetic element that encodes virulence factors and RS1. The B subunit of ctx (ctxB) was reintroduced into the recA gene of the deletion mutants, rendering them unable to recombine with exogenous genetic elements and generating Peru-3, Bang-3, and Bah-3. Fifteen volunteers received one dose of various vaccine strains at 4 x 10(6) to 1 x 10(8) cfu. All strains colonized the gut. A > or = 4-fold rise in vibriocidal titer was observed in 14 volunteers, with titers of > or = 1600 in 13. Peru-3 was the least reactogenic, but 2 of 6 volunteers had loose stools. Peru-14, a filamentous motility-deficient mutant of Peru-3, was well tolerated and colonized 18 of 21 volunteers at doses of 2 x 10(6) to 1 x 10(9) cfu. Also, when 8 Peru-3 or Peru-5 vaccinees, 5 Peru-14 vaccinees, and 8 controls were challenged with 2 x 10(6) cfu V. cholerae El Tor Inaba (N16961), 11 vaccinees were protected compared with no controls. Peru-14 shows promise as a safe, effective, single-dose oral vaccine against El Tor cholera.

Authors+Show Affiliations

Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7995992

Citation

Taylor, D N., et al. "Development of a Live, Oral, Attenuated Vaccine Against El Tor Cholera." The Journal of Infectious Diseases, vol. 170, no. 6, 1994, pp. 1518-23.
Taylor DN, Killeen KP, Hack DC, et al. Development of a live, oral, attenuated vaccine against El Tor cholera. J Infect Dis. 1994;170(6):1518-23.
Taylor, D. N., Killeen, K. P., Hack, D. C., Kenner, J. R., Coster, T. S., Beattie, D. T., Ezzell, J., Hyman, T., Trofa, A., & Sjogren, M. H. (1994). Development of a live, oral, attenuated vaccine against El Tor cholera. The Journal of Infectious Diseases, 170(6), 1518-23.
Taylor DN, et al. Development of a Live, Oral, Attenuated Vaccine Against El Tor Cholera. J Infect Dis. 1994;170(6):1518-23. PubMed PMID: 7995992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a live, oral, attenuated vaccine against El Tor cholera. A1 - Taylor,D N, AU - Killeen,K P, AU - Hack,D C, AU - Kenner,J R, AU - Coster,T S, AU - Beattie,D T, AU - Ezzell,J, AU - Hyman,T, AU - Trofa,A, AU - Sjogren,M H, PY - 1994/12/1/pubmed PY - 1994/12/1/medline PY - 1994/12/1/entrez SP - 1518 EP - 23 JF - The Journal of infectious diseases JO - J. Infect. Dis. VL - 170 IS - 6 N2 - Vibrio cholerae El Tor strains from Peru, Bangladesh, and Bahrain were attenuated by deletion of a genetic element that encodes virulence factors and RS1. The B subunit of ctx (ctxB) was reintroduced into the recA gene of the deletion mutants, rendering them unable to recombine with exogenous genetic elements and generating Peru-3, Bang-3, and Bah-3. Fifteen volunteers received one dose of various vaccine strains at 4 x 10(6) to 1 x 10(8) cfu. All strains colonized the gut. A > or = 4-fold rise in vibriocidal titer was observed in 14 volunteers, with titers of > or = 1600 in 13. Peru-3 was the least reactogenic, but 2 of 6 volunteers had loose stools. Peru-14, a filamentous motility-deficient mutant of Peru-3, was well tolerated and colonized 18 of 21 volunteers at doses of 2 x 10(6) to 1 x 10(9) cfu. Also, when 8 Peru-3 or Peru-5 vaccinees, 5 Peru-14 vaccinees, and 8 controls were challenged with 2 x 10(6) cfu V. cholerae El Tor Inaba (N16961), 11 vaccinees were protected compared with no controls. Peru-14 shows promise as a safe, effective, single-dose oral vaccine against El Tor cholera. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/7995992/Development_of_a_live_oral_attenuated_vaccine_against_El_Tor_cholera_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/170.6.1518 DB - PRIME DP - Unbound Medicine ER -