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Mullerian-inhibiting substance secretion is delayed in XX sex-reversed dog embryos.
Mol Reprod Dev. 1994 Sep; 39(1):1-7.MR

Abstract

Sertoli cell secretion of Mullerian-inhibiting substance (MIS) begins shortly after testis differentiation. Mullerian ducts regress following MIS exposure during an embryonic critical period. In dogs with XX sex reversal, Mullerian ducts persist in the presence of testicular tissue. This study was conducted to determine whether MIS is present in ovotestes of XX sex-reversed embryos during the period for Mullerian duct regression in normal males. XX sex-reversed embryos and normal littermates were identified by a combination of karyotype and gonadal histology. The degree of regression in the adjacent Mullerian duct was scored. Immunohistochemical staining was used to detect MIS in the contralateral gonad. Testicular differentiation and MIS secretion were identified in XY embryos at all ages studied (35-46 days). Seminiferous tubules were not observed in gonads of embryos at risk of XX sex reversal between 35-38 days (n = 15), but were observed at 40 and 46 days (n = 3). Although positive staining for MIS was observed in ovotestes, adjacent Mullerian ducts persisted. The degree of seminiferous tubule development was reduced and MIS secretion was delayed in ovotestes, compared to normal testes. Mullerian duct persistence in this model is apparently due to an abnormality in the quantity and timing of MIS secretion during embryonic development.

Authors+Show Affiliations

L.A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7999353

Citation

Meyers-Wallen, V N., et al. "Mullerian-inhibiting Substance Secretion Is Delayed in XX Sex-reversed Dog Embryos." Molecular Reproduction and Development, vol. 39, no. 1, 1994, pp. 1-7.
Meyers-Wallen VN, MacLaughlin D, Palmer V, et al. Mullerian-inhibiting substance secretion is delayed in XX sex-reversed dog embryos. Mol Reprod Dev. 1994;39(1):1-7.
Meyers-Wallen, V. N., MacLaughlin, D., Palmer, V., & Donahoe, P. K. (1994). Mullerian-inhibiting substance secretion is delayed in XX sex-reversed dog embryos. Molecular Reproduction and Development, 39(1), 1-7.
Meyers-Wallen VN, et al. Mullerian-inhibiting Substance Secretion Is Delayed in XX Sex-reversed Dog Embryos. Mol Reprod Dev. 1994;39(1):1-7. PubMed PMID: 7999353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mullerian-inhibiting substance secretion is delayed in XX sex-reversed dog embryos. AU - Meyers-Wallen,V N, AU - MacLaughlin,D, AU - Palmer,V, AU - Donahoe,P K, PY - 1994/9/1/pubmed PY - 1994/9/1/medline PY - 1994/9/1/entrez SP - 1 EP - 7 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 39 IS - 1 N2 - Sertoli cell secretion of Mullerian-inhibiting substance (MIS) begins shortly after testis differentiation. Mullerian ducts regress following MIS exposure during an embryonic critical period. In dogs with XX sex reversal, Mullerian ducts persist in the presence of testicular tissue. This study was conducted to determine whether MIS is present in ovotestes of XX sex-reversed embryos during the period for Mullerian duct regression in normal males. XX sex-reversed embryos and normal littermates were identified by a combination of karyotype and gonadal histology. The degree of regression in the adjacent Mullerian duct was scored. Immunohistochemical staining was used to detect MIS in the contralateral gonad. Testicular differentiation and MIS secretion were identified in XY embryos at all ages studied (35-46 days). Seminiferous tubules were not observed in gonads of embryos at risk of XX sex reversal between 35-38 days (n = 15), but were observed at 40 and 46 days (n = 3). Although positive staining for MIS was observed in ovotestes, adjacent Mullerian ducts persisted. The degree of seminiferous tubule development was reduced and MIS secretion was delayed in ovotestes, compared to normal testes. Mullerian duct persistence in this model is apparently due to an abnormality in the quantity and timing of MIS secretion during embryonic development. SN - 1040-452X UR - https://www.unboundmedicine.com/medline/citation/7999353/Mullerian_inhibiting_substance_secretion_is_delayed_in_XX_sex_reversed_dog_embryos_ L2 - https://doi.org/10.1002/mrd.1080390102 DB - PRIME DP - Unbound Medicine ER -