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Characterization of CA125 glycoprotein using 3 monoclonal antibodies with different specificities.
Gynecol Obstet Invest. 1994; 37(3):199-203.GO

Abstract

CA125 and CA130 in various tissues were characterized using the monoclonal antibodies OC125, 130-22, and 145-9. CA125 and CA130 were eluted in the same fraction in 130-22 affinity chromatography. A sandwich immunoradiometric assay using these antibodies in combination was established. When the solid phase of the assay was changed from OC125 to 145-9, the amount of 125I-OC125 that reacted with CA125 in the amnion increased more than that in other samples. On SDS-PAGE, CA125 and CA130 epitopes in the amnion migrated to different positions. These results indicate that CA125 and CA130 are located on different subunits of the same glycoprotein, and that their synthesis is regulated differently in various tissues.

Authors+Show Affiliations

Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8005553

Citation

Kobayashi, F, et al. "Characterization of CA125 Glycoprotein Using 3 Monoclonal Antibodies With Different Specificities." Gynecologic and Obstetric Investigation, vol. 37, no. 3, 1994, pp. 199-203.
Kobayashi F, Sagawa N, Fujii S, et al. Characterization of CA125 glycoprotein using 3 monoclonal antibodies with different specificities. Gynecol Obstet Invest. 1994;37(3):199-203.
Kobayashi, F., Sagawa, N., Fujii, S., Mori, T., & Endo, K. (1994). Characterization of CA125 glycoprotein using 3 monoclonal antibodies with different specificities. Gynecologic and Obstetric Investigation, 37(3), 199-203.
Kobayashi F, et al. Characterization of CA125 Glycoprotein Using 3 Monoclonal Antibodies With Different Specificities. Gynecol Obstet Invest. 1994;37(3):199-203. PubMed PMID: 8005553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of CA125 glycoprotein using 3 monoclonal antibodies with different specificities. AU - Kobayashi,F, AU - Sagawa,N, AU - Fujii,S, AU - Mori,T, AU - Endo,K, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 199 EP - 203 JF - Gynecologic and obstetric investigation JO - Gynecol Obstet Invest VL - 37 IS - 3 N2 - CA125 and CA130 in various tissues were characterized using the monoclonal antibodies OC125, 130-22, and 145-9. CA125 and CA130 were eluted in the same fraction in 130-22 affinity chromatography. A sandwich immunoradiometric assay using these antibodies in combination was established. When the solid phase of the assay was changed from OC125 to 145-9, the amount of 125I-OC125 that reacted with CA125 in the amnion increased more than that in other samples. On SDS-PAGE, CA125 and CA130 epitopes in the amnion migrated to different positions. These results indicate that CA125 and CA130 are located on different subunits of the same glycoprotein, and that their synthesis is regulated differently in various tissues. SN - 0378-7346 UR - https://www.unboundmedicine.com/medline/citation/8005553/Characterization_of_CA125_glycoprotein_using_3_monoclonal_antibodies_with_different_specificities_ L2 - https://www.karger.com?DOI=10.1159/000292559 DB - PRIME DP - Unbound Medicine ER -