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Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver.
J Lipid Res. 1994 Apr; 35(4):709-20.JL

Abstract

The participation of hepatic lipase in the initial uptake and processing of chylomicron remnants was studied in the isolated, perfused rat liver. Hepatic lipase activity was either reduced by preperfusion of livers with heparin or inhibited with specific rat hepatic lipase antibodies. [3H]palmitate-labeled chylomicron remnants were recirculated through control and treated livers for 15 min; the livers were then flushed, homogenized, and endosome-rich fractions were isolated. Depletion of hepatic lipase activity by both methods reduced the uptake of chylomicron remnants and hydrolysis of their component triglycerides by perfused rat livers, but at the same time significantly increased the rate of endocytosis of those chylomicron remnants taken up. We conclude that hepatic lipase facilitates, but is not essential for, the initial uptake of chylomicron remnants by rat liver. Furthermore, endocytosis of chylomicron remnants does not require binding to hepatic lipase or the associated hydrolysis of remnant lipids.

Authors+Show Affiliations

Cardiovascular Research Institute, University of California, San Francisco 94143-0130.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8006526

Citation

Shafi, S, et al. "Role of Hepatic Lipase in the Uptake and Processing of Chylomicron Remnants in Rat Liver." Journal of Lipid Research, vol. 35, no. 4, 1994, pp. 709-20.
Shafi S, Brady SE, Bensadoun A, et al. Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver. J Lipid Res. 1994;35(4):709-20.
Shafi, S., Brady, S. E., Bensadoun, A., & Havel, R. J. (1994). Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver. Journal of Lipid Research, 35(4), 709-20.
Shafi S, et al. Role of Hepatic Lipase in the Uptake and Processing of Chylomicron Remnants in Rat Liver. J Lipid Res. 1994;35(4):709-20. PubMed PMID: 8006526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver. AU - Shafi,S, AU - Brady,S E, AU - Bensadoun,A, AU - Havel,R J, PY - 1994/4/1/pubmed PY - 1994/4/1/medline PY - 1994/4/1/entrez SP - 709 EP - 20 JF - Journal of lipid research JO - J Lipid Res VL - 35 IS - 4 N2 - The participation of hepatic lipase in the initial uptake and processing of chylomicron remnants was studied in the isolated, perfused rat liver. Hepatic lipase activity was either reduced by preperfusion of livers with heparin or inhibited with specific rat hepatic lipase antibodies. [3H]palmitate-labeled chylomicron remnants were recirculated through control and treated livers for 15 min; the livers were then flushed, homogenized, and endosome-rich fractions were isolated. Depletion of hepatic lipase activity by both methods reduced the uptake of chylomicron remnants and hydrolysis of their component triglycerides by perfused rat livers, but at the same time significantly increased the rate of endocytosis of those chylomicron remnants taken up. We conclude that hepatic lipase facilitates, but is not essential for, the initial uptake of chylomicron remnants by rat liver. Furthermore, endocytosis of chylomicron remnants does not require binding to hepatic lipase or the associated hydrolysis of remnant lipids. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/8006526/Role_of_hepatic_lipase_in_the_uptake_and_processing_of_chylomicron_remnants_in_rat_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2275(20)41185-X DB - PRIME DP - Unbound Medicine ER -