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Urokinase-type and tissue-type plasminogen activators as growth factors of human fibroblasts.
Exp Cell Res. 1994 Jul; 213(1):286-94.EC

Abstract

In this study we have verified the mitogenic effect of urokinase-type (u-PA) and tissue-type plasminogen activators (t-PA) on human normal fibroblasts. We report that both PAs can induce DNA replication and cell division in serum-deprived cultured human skin fibroblasts. The activity of u-PA and t-PA is, respectively, three- and twofold more potent than that exerted by epidermal growth factor (EGF) with an activity slightly lower (50-60%) than that induced by basic fibroblast growth factor (bFGF). The u-PA and t-PA, but not plasmin, induced DNA synthesis, which could be neutralized by anti-u-PA and anti-t-PA antibodies, respectively, but was insensitive to aprotinin treatment. The addition of anti-u-PA-receptor (u-PAR) monoclonal antibodies to the assays selectively suppressed the mitogenic effect exerted by u-PA, but not that of t-PA, and the amino-terminal fragment of u-PA, containing the EGF-like domain and the kringle module, did not elicit any mitogenic activity. Anti-bFGF antibodies completely suppressed the mitogenic activity of bFGF, but did not have any effect on that of u-PA and t-PA; the activity of both PAs was inhibited by anti-fibronectin IgG concentrations ineffective on bFGF. These results indicate that PAs may be considered growth factors of human fibroblasts.

Authors+Show Affiliations

Department of Biomedical Sciences and Biotechnologies, University of Brescia, Italy.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8020601

Citation

De Petro, G, et al. "Urokinase-type and Tissue-type Plasminogen Activators as Growth Factors of Human Fibroblasts." Experimental Cell Research, vol. 213, no. 1, 1994, pp. 286-94.
De Petro G, Copeta A, Barlati S. Urokinase-type and tissue-type plasminogen activators as growth factors of human fibroblasts. Exp Cell Res. 1994;213(1):286-94.
De Petro, G., Copeta, A., & Barlati, S. (1994). Urokinase-type and tissue-type plasminogen activators as growth factors of human fibroblasts. Experimental Cell Research, 213(1), 286-94.
De Petro G, Copeta A, Barlati S. Urokinase-type and Tissue-type Plasminogen Activators as Growth Factors of Human Fibroblasts. Exp Cell Res. 1994;213(1):286-94. PubMed PMID: 8020601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urokinase-type and tissue-type plasminogen activators as growth factors of human fibroblasts. AU - De Petro,G, AU - Copeta,A, AU - Barlati,S, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 286 EP - 94 JF - Experimental cell research JO - Exp Cell Res VL - 213 IS - 1 N2 - In this study we have verified the mitogenic effect of urokinase-type (u-PA) and tissue-type plasminogen activators (t-PA) on human normal fibroblasts. We report that both PAs can induce DNA replication and cell division in serum-deprived cultured human skin fibroblasts. The activity of u-PA and t-PA is, respectively, three- and twofold more potent than that exerted by epidermal growth factor (EGF) with an activity slightly lower (50-60%) than that induced by basic fibroblast growth factor (bFGF). The u-PA and t-PA, but not plasmin, induced DNA synthesis, which could be neutralized by anti-u-PA and anti-t-PA antibodies, respectively, but was insensitive to aprotinin treatment. The addition of anti-u-PA-receptor (u-PAR) monoclonal antibodies to the assays selectively suppressed the mitogenic effect exerted by u-PA, but not that of t-PA, and the amino-terminal fragment of u-PA, containing the EGF-like domain and the kringle module, did not elicit any mitogenic activity. Anti-bFGF antibodies completely suppressed the mitogenic activity of bFGF, but did not have any effect on that of u-PA and t-PA; the activity of both PAs was inhibited by anti-fibronectin IgG concentrations ineffective on bFGF. These results indicate that PAs may be considered growth factors of human fibroblasts. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/8020601/Urokinase_type_and_tissue_type_plasminogen_activators_as_growth_factors_of_human_fibroblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(84)71200-6 DB - PRIME DP - Unbound Medicine ER -