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SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane.
J Bacteriol 1994; 176(13):4111-6JB

Abstract

The effect of the overproduction of SecF encoded by the tac-secF gene on a plasmid on the synthesis of other Sec proteins was studied in Escherichia coli. SecF overproduction resulted in the simultaneous overproduction of SecD encoded by the tac-secD gene on a plasmid. Deletion of the orf6 gene, located downstream of the secF gene, had no effect on SecD overproduction. A pulse-chase experiment revealed that the overproduction was due to stabilization of SecD with SecF. SecF overproduction also resulted in the overproduction of SecY encoded by the tac-secY gene on a plasmid as well. SecF overproduction also enhanced the level of SecY expressed by the chromosomal secY gene. This SecF effect was not due to its effect on SecD or SecE, since SecF overproduction did not affect the levels of SecD and SecE expressed by the chromosomal secD and secE genes, respectively. SecE-dependent overproduction of SecY has already been demonstrated. It is suggested that SecF interacts with both SecD and SecY. SecE-SecY interaction has been demonstrated. It is likely, therefore, that all Sec proteins in the cytoplasmic membrane interact with each other.

Authors+Show Affiliations

Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8021192

Citation

Sagara, K, et al. "SecF Stabilizes SecD and SecY, Components of the Protein Translocation Machinery of the Escherichia Coli Cytoplasmic Membrane." Journal of Bacteriology, vol. 176, no. 13, 1994, pp. 4111-6.
Sagara K, Matsuyama S, Mizushima S. SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane. J Bacteriol. 1994;176(13):4111-6.
Sagara, K., Matsuyama, S., & Mizushima, S. (1994). SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane. Journal of Bacteriology, 176(13), pp. 4111-6.
Sagara K, Matsuyama S, Mizushima S. SecF Stabilizes SecD and SecY, Components of the Protein Translocation Machinery of the Escherichia Coli Cytoplasmic Membrane. J Bacteriol. 1994;176(13):4111-6. PubMed PMID: 8021192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane. AU - Sagara,K, AU - Matsuyama,S, AU - Mizushima,S, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 4111 EP - 6 JF - Journal of bacteriology JO - J. Bacteriol. VL - 176 IS - 13 N2 - The effect of the overproduction of SecF encoded by the tac-secF gene on a plasmid on the synthesis of other Sec proteins was studied in Escherichia coli. SecF overproduction resulted in the simultaneous overproduction of SecD encoded by the tac-secD gene on a plasmid. Deletion of the orf6 gene, located downstream of the secF gene, had no effect on SecD overproduction. A pulse-chase experiment revealed that the overproduction was due to stabilization of SecD with SecF. SecF overproduction also resulted in the overproduction of SecY encoded by the tac-secY gene on a plasmid as well. SecF overproduction also enhanced the level of SecY expressed by the chromosomal secY gene. This SecF effect was not due to its effect on SecD or SecE, since SecF overproduction did not affect the levels of SecD and SecE expressed by the chromosomal secD and secE genes, respectively. SecE-dependent overproduction of SecY has already been demonstrated. It is suggested that SecF interacts with both SecD and SecY. SecE-SecY interaction has been demonstrated. It is likely, therefore, that all Sec proteins in the cytoplasmic membrane interact with each other. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8021192/SecF_stabilizes_SecD_and_SecY_components_of_the_protein_translocation_machinery_of_the_Escherichia_coli_cytoplasmic_membrane_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8021192 DB - PRIME DP - Unbound Medicine ER -