Tags

Type your tag names separated by a space and hit enter

Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate.
Mol Microbiol 1994; 11(6):1099-108MM

Abstract

Transposition of conjugative transposons proceeds by excision and formation of a covalently closed circular intermediate that includes at its joint the six flanking bases from its previous host (coupling sequences). To elucidate the role of the coupling sequences in this process and to determine the sequence of targets used by Tn916, we studied its insertion into a plasmid following conjugation. The results differ from those previously observed when Tn916 was introduced by transformation. They suggest that only one specific strand of the transposon molecule is transferred during the conjugation event and that complementary strand synthesis produces a double-stranded transposon circle with no mismatches which serves as the reaction intermediate. Tn916 inserts preferentially at specific sites and the same targets are used when Tn916 comes from donors with different coupling sequences. An analysis of the sequences of preferred targets is presented.

Authors+Show Affiliations

Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8022279

Citation

Scott, J R., et al. "Conjugative Transposition of Tn916: Preferred Targets and Evidence for Conjugative Transfer of a Single Strand and for a Double-stranded Circular Intermediate." Molecular Microbiology, vol. 11, no. 6, 1994, pp. 1099-108.
Scott JR, Bringel F, Marra D, et al. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol Microbiol. 1994;11(6):1099-108.
Scott, J. R., Bringel, F., Marra, D., Van Alstine, G., & Rudy, C. K. (1994). Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Molecular Microbiology, 11(6), pp. 1099-108.
Scott JR, et al. Conjugative Transposition of Tn916: Preferred Targets and Evidence for Conjugative Transfer of a Single Strand and for a Double-stranded Circular Intermediate. Mol Microbiol. 1994;11(6):1099-108. PubMed PMID: 8022279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. AU - Scott,J R, AU - Bringel,F, AU - Marra,D, AU - Van Alstine,G, AU - Rudy,C K, PY - 1994/3/1/pubmed PY - 1994/3/1/medline PY - 1994/3/1/entrez SP - 1099 EP - 108 JF - Molecular microbiology JO - Mol. Microbiol. VL - 11 IS - 6 N2 - Transposition of conjugative transposons proceeds by excision and formation of a covalently closed circular intermediate that includes at its joint the six flanking bases from its previous host (coupling sequences). To elucidate the role of the coupling sequences in this process and to determine the sequence of targets used by Tn916, we studied its insertion into a plasmid following conjugation. The results differ from those previously observed when Tn916 was introduced by transformation. They suggest that only one specific strand of the transposon molecule is transferred during the conjugation event and that complementary strand synthesis produces a double-stranded transposon circle with no mismatches which serves as the reaction intermediate. Tn916 inserts preferentially at specific sites and the same targets are used when Tn916 comes from donors with different coupling sequences. An analysis of the sequences of preferred targets is presented. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/8022279/Conjugative_transposition_of_Tn916:_preferred_targets_and_evidence_for_conjugative_transfer_of_a_single_strand_and_for_a_double_stranded_circular_intermediate_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1994&volume=11&issue=6&spage=1099 DB - PRIME DP - Unbound Medicine ER -