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Mechanism of histamine release from rat mast cells induced by the ionophore A23187: effects of calcium and temperature.
Br J Pharmacol. 1978 Aug; 63(4):643-9.BJ

Abstract

1 The mechanism of histamine release from a pure population of rat mast cells induced by the lipid soluble antibiotic, A23187, has been studied and compared with data for anaphylactic histamine release reported in the literature. 2 Histamine release induced by A23187 in the presence of calcium 10(-3) mol/l was completed in 10 minutes. By preincubation of the mast cells with A23187 for 10 min in the absence of calcium the histamine release induced by calcium, 10(-3) mol/l or 5 x 10(-3) mol/l, was completed in 90 s and 45 s, respectively. 3 A23187-induced histamine release was maximal with calcium 10(-3) mol/l when the cells were incubated at 33 to 39 degrees C for 10 minutes. 4 The cellular mechanism, which was stimulated by A23187 and calcium for the release of histamine, was irreversibly inactivated by incubation at 45 degrees C. 5 An inhibition of energy metabolism was excluded as the cause of the heat inactivation. 6 The dependence of A23187-induced histamine release on calcium and temperature, the time course of histamine release and the heat inactivation are consistent with the view that the same mechanism is involved in A23187-induced and anaphylactic histamine release.

Authors

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

80244

Citation

Johansen, T. "Mechanism of Histamine Release From Rat Mast Cells Induced By the Ionophore A23187: Effects of Calcium and Temperature." British Journal of Pharmacology, vol. 63, no. 4, 1978, pp. 643-9.
Johansen T. Mechanism of histamine release from rat mast cells induced by the ionophore A23187: effects of calcium and temperature. Br J Pharmacol. 1978;63(4):643-9.
Johansen, T. (1978). Mechanism of histamine release from rat mast cells induced by the ionophore A23187: effects of calcium and temperature. British Journal of Pharmacology, 63(4), 643-9.
Johansen T. Mechanism of Histamine Release From Rat Mast Cells Induced By the Ionophore A23187: Effects of Calcium and Temperature. Br J Pharmacol. 1978;63(4):643-9. PubMed PMID: 80244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of histamine release from rat mast cells induced by the ionophore A23187: effects of calcium and temperature. A1 - Johansen,T, PY - 1978/8/1/pubmed PY - 1978/8/1/medline PY - 1978/8/1/entrez SP - 643 EP - 9 JF - British journal of pharmacology JO - Br J Pharmacol VL - 63 IS - 4 N2 - 1 The mechanism of histamine release from a pure population of rat mast cells induced by the lipid soluble antibiotic, A23187, has been studied and compared with data for anaphylactic histamine release reported in the literature. 2 Histamine release induced by A23187 in the presence of calcium 10(-3) mol/l was completed in 10 minutes. By preincubation of the mast cells with A23187 for 10 min in the absence of calcium the histamine release induced by calcium, 10(-3) mol/l or 5 x 10(-3) mol/l, was completed in 90 s and 45 s, respectively. 3 A23187-induced histamine release was maximal with calcium 10(-3) mol/l when the cells were incubated at 33 to 39 degrees C for 10 minutes. 4 The cellular mechanism, which was stimulated by A23187 and calcium for the release of histamine, was irreversibly inactivated by incubation at 45 degrees C. 5 An inhibition of energy metabolism was excluded as the cause of the heat inactivation. 6 The dependence of A23187-induced histamine release on calcium and temperature, the time course of histamine release and the heat inactivation are consistent with the view that the same mechanism is involved in A23187-induced and anaphylactic histamine release. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/80244/Mechanism_of_histamine_release_from_rat_mast_cells_induced_by_the_ionophore_A23187:_effects_of_calcium_and_temperature_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1188&date=1978&volume=63&issue=4&spage=643 DB - PRIME DP - Unbound Medicine ER -