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Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.
Microbiology (Reading). 1994 May; 140 (Pt 5):1045-1050.M

Abstract

Various Streptomyces spp. including S. violaceus, S. scabies, S. griseus, S. exfoliatus, S. coelicolor and S. lividans secrete indole-3-acetic acid (IAA) when fed with L-tryptophan (Trp). Production of IAA was detected in Streptomyces strains causing potato scab as well as in non-pathogenic strains. The pathways for IAA synthesis from Trp were investigated in S. violaceus and S. exfoliatus. Indole-3-acetamide (IAM), indole-3-lactic acid (ILA), indole-3-ethanol (IEt) and IAA were identified by HPLC and GC-MS. Streptomyces cells were capable of catabolizing IAM, ILA, IEt and indole-3-acetaldehyde (IAAId) into IAA. Incorporation of radioactivity into IAM, IAA and ILA but not IEt was detected when cells were fed with L-[3-14C]tryptophan. Results indicate the presence of the IAM pathway (Trp-->IAM-->IAA) and the possible presence of additional pathways for IAA biosynthesis in Streptomyces.

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Authors

Manulis S
No affiliation info available
Shafrir H
No affiliation info available
Epstein E
No affiliation info available
Lichter A
No affiliation info available
Barash I
No affiliation info available

MeSH

Chromatography, High Pressure LiquidGas Chromatography-Mass SpectrometryIndoleacetic AcidsIndolesModels, BiologicalPlant DiseasesSpecies SpecificityStreptomycesTryptophanVirulence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

8025670

Citation

Manulis, Shulamit, et al. "Biosynthesis of Indole-3-acetic Acid Via the Indole-3-acetamide Pathway in Streptomyces Spp." Microbiology (Reading, England), vol. 140 (Pt 5), 1994, pp. 1045-1050.
Manulis S, Shafrir H, Epstein E, et al. Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. Microbiology (Reading). 1994;140 (Pt 5):1045-1050.
Manulis, S., Shafrir, H., Epstein, E., Lichter, A., & Barash, I. (1994). Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. Microbiology (Reading, England), 140 (Pt 5), 1045-1050. https://doi.org/10.1099/13500872-140-5-1045
Manulis S, et al. Biosynthesis of Indole-3-acetic Acid Via the Indole-3-acetamide Pathway in Streptomyces Spp. Microbiology (Reading). 1994;140 (Pt 5):1045-1050. PubMed PMID: 8025670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. AU - Manulis,Shulamit, AU - Shafrir,Hadas, AU - Epstein,Ephraim, AU - Lichter,Amnon, AU - Barash,Isaac, PY - 1994/5/1/pubmed PY - 1994/5/1/medline PY - 1994/5/1/entrez SP - 1045 EP - 1050 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 140 (Pt 5) N2 - Various Streptomyces spp. including S. violaceus, S. scabies, S. griseus, S. exfoliatus, S. coelicolor and S. lividans secrete indole-3-acetic acid (IAA) when fed with L-tryptophan (Trp). Production of IAA was detected in Streptomyces strains causing potato scab as well as in non-pathogenic strains. The pathways for IAA synthesis from Trp were investigated in S. violaceus and S. exfoliatus. Indole-3-acetamide (IAM), indole-3-lactic acid (ILA), indole-3-ethanol (IEt) and IAA were identified by HPLC and GC-MS. Streptomyces cells were capable of catabolizing IAM, ILA, IEt and indole-3-acetaldehyde (IAAId) into IAA. Incorporation of radioactivity into IAM, IAA and ILA but not IEt was detected when cells were fed with L-[3-14C]tryptophan. Results indicate the presence of the IAM pathway (Trp-->IAM-->IAA) and the possible presence of additional pathways for IAA biosynthesis in Streptomyces. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/8025670/Biosynthesis_of_indole_3_acetic_acid_via_the_indole_3_acetamide_pathway_in_Streptomyces_spp_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/13500872-140-5-1045 DB - PRIME DP - Unbound Medicine ER -