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ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter.
Gene 1994; 144(1):59-62GENE

Abstract

A new family of T7-based expression plasmids with unique features is described. The plasmid origin of replication (ori), derived from P15A, is compatible with that of ColE1-derived plasmids, which facilitates the co-production of proteins from these vectors and from ColE1-derived T7 expression vectors in the same cell. The plasmids are medium-copy-number and also carry the M13 ori. Consequently, both double- and single-stranded DNA can be easily obtained. The plasmids encode KmR, thus avoiding the potential for plasmid loss associated with ApR-based systems. One of the plasmids carries the lacI gene, to allow for more stringent regulation of the production of potentially toxic proteins. When the plasmids are introduced into an Escherichia coli strain such as BL21(DE3), which contains the T7 polymerase-encoding gene under control of the lacUV5 promoter, addition of IPTG initiates the production of high levels of the recombinant protein.

Authors+Show Affiliations

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8026759

Citation

Munson, M, et al. "ColE1-compatible Vectors for High-level Expression of Cloned DNAs From the T7 Promoter." Gene, vol. 144, no. 1, 1994, pp. 59-62.
Munson M, Predki PF, Regan L. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene. 1994;144(1):59-62.
Munson, M., Predki, P. F., & Regan, L. (1994). ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene, 144(1), pp. 59-62.
Munson M, Predki PF, Regan L. ColE1-compatible Vectors for High-level Expression of Cloned DNAs From the T7 Promoter. Gene. 1994 Jun 24;144(1):59-62. PubMed PMID: 8026759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. AU - Munson,M, AU - Predki,P F, AU - Regan,L, PY - 1994/6/24/pubmed PY - 1994/6/24/medline PY - 1994/6/24/entrez SP - 59 EP - 62 JF - Gene JO - Gene VL - 144 IS - 1 N2 - A new family of T7-based expression plasmids with unique features is described. The plasmid origin of replication (ori), derived from P15A, is compatible with that of ColE1-derived plasmids, which facilitates the co-production of proteins from these vectors and from ColE1-derived T7 expression vectors in the same cell. The plasmids are medium-copy-number and also carry the M13 ori. Consequently, both double- and single-stranded DNA can be easily obtained. The plasmids encode KmR, thus avoiding the potential for plasmid loss associated with ApR-based systems. One of the plasmids carries the lacI gene, to allow for more stringent regulation of the production of potentially toxic proteins. When the plasmids are introduced into an Escherichia coli strain such as BL21(DE3), which contains the T7 polymerase-encoding gene under control of the lacUV5 promoter, addition of IPTG initiates the production of high levels of the recombinant protein. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/8026759/ColE1_compatible_vectors_for_high_level_expression_of_cloned_DNAs_from_the_T7_promoter_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(94)90203-8 DB - PRIME DP - Unbound Medicine ER -