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Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
J Immunol. 1994 Aug 01; 153(3):979-86.JI

Abstract

A mAb called V65 was raised to a CD3+, TCR-alpha/beta- rat/mouse T cell hybrid that selectively reacts with all CD3+, TCR-alpha/beta- rat lymphocytes. Both anti-CD3 and V65 precipitate a 48- to 50-kDa heterodimeric protein from digitonin-lysed surface-iodinated cells. V65+ but not V65- T cells and T cell hybridoma cells express TCR-gamma mRNA. Together, these results show that V65 detects a constant determinant of the rat TCR-gamma/delta. In the presence of either IL-2 or IL-4, V65 stimulates proliferation in peripheral rat gamma/delta T cells. Approximately 90% of gamma/delta T cells from peripheral lymphoid organs have the same cell surface phenotype as thymus-derived MHC class I-restricted alpha/beta T cells, i.e., they are CD4- but express the CD8 alpha/beta heterodimer together with CD2 and CD5. In contrast, gamma/delta T cells from the epithelium of the small intestine lack CD2, CD4, and CD5 and express CD8 alpha only. Finally, V65 directly identifies a dense network of dendritic cells in the epidermis as gamma/delta T cells. These dendritic epidermal T cells are absent from athymic rats, indicating that like their mouse counterparts, they are thymus dependent.

Authors+Show Affiliations

Institute of Virology and Immunobiology, University of Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8027567

Citation

Kühnlein, P, et al. "Identification and Characterization of Rat Gamma/delta T Lymphocytes in Peripheral Lymphoid Organs, Small Intestine, and Skin With a Monoclonal Antibody to a Constant Determinant of the Gamma/delta T Cell Receptor." Journal of Immunology (Baltimore, Md. : 1950), vol. 153, no. 3, 1994, pp. 979-86.
Kühnlein P, Park JH, Herrmann T, et al. Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994;153(3):979-86.
Kühnlein, P., Park, J. H., Herrmann, T., Elbe, A., & Hünig, T. (1994). Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. Journal of Immunology (Baltimore, Md. : 1950), 153(3), 979-86.
Kühnlein P, et al. Identification and Characterization of Rat Gamma/delta T Lymphocytes in Peripheral Lymphoid Organs, Small Intestine, and Skin With a Monoclonal Antibody to a Constant Determinant of the Gamma/delta T Cell Receptor. J Immunol. 1994 Aug 1;153(3):979-86. PubMed PMID: 8027567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. AU - Kühnlein,P, AU - Park,J H, AU - Herrmann,T, AU - Elbe,A, AU - Hünig,T, PY - 1994/8/1/pubmed PY - 1994/8/1/medline PY - 1994/8/1/entrez SP - 979 EP - 86 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 153 IS - 3 N2 - A mAb called V65 was raised to a CD3+, TCR-alpha/beta- rat/mouse T cell hybrid that selectively reacts with all CD3+, TCR-alpha/beta- rat lymphocytes. Both anti-CD3 and V65 precipitate a 48- to 50-kDa heterodimeric protein from digitonin-lysed surface-iodinated cells. V65+ but not V65- T cells and T cell hybridoma cells express TCR-gamma mRNA. Together, these results show that V65 detects a constant determinant of the rat TCR-gamma/delta. In the presence of either IL-2 or IL-4, V65 stimulates proliferation in peripheral rat gamma/delta T cells. Approximately 90% of gamma/delta T cells from peripheral lymphoid organs have the same cell surface phenotype as thymus-derived MHC class I-restricted alpha/beta T cells, i.e., they are CD4- but express the CD8 alpha/beta heterodimer together with CD2 and CD5. In contrast, gamma/delta T cells from the epithelium of the small intestine lack CD2, CD4, and CD5 and express CD8 alpha only. Finally, V65 directly identifies a dense network of dendritic cells in the epidermis as gamma/delta T cells. These dendritic epidermal T cells are absent from athymic rats, indicating that like their mouse counterparts, they are thymus dependent. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8027567/Identification_and_characterization_of_rat_gamma/delta_T_lymphocytes_in_peripheral_lymphoid_organs_small_intestine_and_skin_with_a_monoclonal_antibody_to_a_constant_determinant_of_the_gamma/delta_T_cell_receptor_ DB - PRIME DP - Unbound Medicine ER -