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The anti-infarct effect of an adenosine A1-selective agonist is diminished after prolonged infusion as is the cardioprotective effect of ischaemic preconditioning in rabbit heart.
J Mol Cell Cardiol. 1994 Mar; 26(3):303-11.JM

Abstract

Our aim was to determine whether adenosine A1 receptor-mediated protection could be maintained for a prolonged period of time by a continuous infusion of an A1-selective agonist. To produce myocardial infarction a branch of the left coronary artery of rabbit hearts was occluded for 30 min and reperfused for 3 h. Infarct size was determined with tetrazolium staining. Prior to the 30 min ischaemia, rabbits were subjected to one of the following six protocols: (1) 6 h i.v. saline infusion; (2) 6 h i.v. CCPA (0.043 mg/kg/h) infusion; (3) 72 h saline infusion; (4) 72 h CCPA infusion; (5) 72 h CCPA infusion plus preconditioning with 5 min ischaemia followed by 10 min reperfusion; (6) 72 h saline infusion plus preconditioning. The 6 h CCPA infusion group had significantly smaller infarct sizes than the 6 h vehicle group. 16.2 +/- 2.9% infarction of the ischaemic region v 39.5 +/- 2.6%, P < 0.01. Infarction in the 72 h CCPA infusion group (37.7 +/- 2.7%) was the same as in the 72 h vehicle group (35.2 +/- 3.1%). Ischaemic preconditioning could not limit infarct size in 72 h CCPA animals (%infarction; 29.1 +/- 4.6%) but did protect animals given vehicle for 72 h (8.4 +/- 1.2%, P < 0.01). After 72 h infusion of CCPA, both the cardioprotective effect of adenosine A1-selective agonist and ischaemic preconditioning were attenuated. These findings indicate that: (1) the myocytes become desensitized to the protective effect of CCPA with prolonged exposure; and (2) ischaemic preconditioning is no longer protective when tachyphylaxis to CCPA occurs.

Authors+Show Affiliations

Department of Physiology, University of South Alabama, Mobile 36688.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8028014

Citation

Tsuchida, A, et al. "The Anti-infarct Effect of an Adenosine A1-selective Agonist Is Diminished After Prolonged Infusion as Is the Cardioprotective Effect of Ischaemic Preconditioning in Rabbit Heart." Journal of Molecular and Cellular Cardiology, vol. 26, no. 3, 1994, pp. 303-11.
Tsuchida A, Thompson R, Olsson RA, et al. The anti-infarct effect of an adenosine A1-selective agonist is diminished after prolonged infusion as is the cardioprotective effect of ischaemic preconditioning in rabbit heart. J Mol Cell Cardiol. 1994;26(3):303-11.
Tsuchida, A., Thompson, R., Olsson, R. A., & Downey, J. M. (1994). The anti-infarct effect of an adenosine A1-selective agonist is diminished after prolonged infusion as is the cardioprotective effect of ischaemic preconditioning in rabbit heart. Journal of Molecular and Cellular Cardiology, 26(3), 303-11.
Tsuchida A, et al. The Anti-infarct Effect of an Adenosine A1-selective Agonist Is Diminished After Prolonged Infusion as Is the Cardioprotective Effect of Ischaemic Preconditioning in Rabbit Heart. J Mol Cell Cardiol. 1994;26(3):303-11. PubMed PMID: 8028014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The anti-infarct effect of an adenosine A1-selective agonist is diminished after prolonged infusion as is the cardioprotective effect of ischaemic preconditioning in rabbit heart. AU - Tsuchida,A, AU - Thompson,R, AU - Olsson,R A, AU - Downey,J M, PY - 1994/3/1/pubmed PY - 1994/3/1/medline PY - 1994/3/1/entrez SP - 303 EP - 11 JF - Journal of molecular and cellular cardiology JO - J Mol Cell Cardiol VL - 26 IS - 3 N2 - Our aim was to determine whether adenosine A1 receptor-mediated protection could be maintained for a prolonged period of time by a continuous infusion of an A1-selective agonist. To produce myocardial infarction a branch of the left coronary artery of rabbit hearts was occluded for 30 min and reperfused for 3 h. Infarct size was determined with tetrazolium staining. Prior to the 30 min ischaemia, rabbits were subjected to one of the following six protocols: (1) 6 h i.v. saline infusion; (2) 6 h i.v. CCPA (0.043 mg/kg/h) infusion; (3) 72 h saline infusion; (4) 72 h CCPA infusion; (5) 72 h CCPA infusion plus preconditioning with 5 min ischaemia followed by 10 min reperfusion; (6) 72 h saline infusion plus preconditioning. The 6 h CCPA infusion group had significantly smaller infarct sizes than the 6 h vehicle group. 16.2 +/- 2.9% infarction of the ischaemic region v 39.5 +/- 2.6%, P < 0.01. Infarction in the 72 h CCPA infusion group (37.7 +/- 2.7%) was the same as in the 72 h vehicle group (35.2 +/- 3.1%). Ischaemic preconditioning could not limit infarct size in 72 h CCPA animals (%infarction; 29.1 +/- 4.6%) but did protect animals given vehicle for 72 h (8.4 +/- 1.2%, P < 0.01). After 72 h infusion of CCPA, both the cardioprotective effect of adenosine A1-selective agonist and ischaemic preconditioning were attenuated. These findings indicate that: (1) the myocytes become desensitized to the protective effect of CCPA with prolonged exposure; and (2) ischaemic preconditioning is no longer protective when tachyphylaxis to CCPA occurs. SN - 0022-2828 UR - https://www.unboundmedicine.com/medline/citation/8028014/The_anti_infarct_effect_of_an_adenosine_A1_selective_agonist_is_diminished_after_prolonged_infusion_as_is_the_cardioprotective_effect_of_ischaemic_preconditioning_in_rabbit_heart_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2828(84)71039-X DB - PRIME DP - Unbound Medicine ER -