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Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium.
Mol Gen Genet. 1994 Jun 15; 243(6):605-12.MG

Abstract

More than 50 genes are required for flagellar formation and function in Salmonella typhimurium. According to the cascade model of the flagellar regulon, the flagellar operons are divided into three classes, 1, 2, and 3, with reference to their relative positions in the transcriptional hierarchy. This sequential transcription is coupled to the assembly process of the flagellar structure, that is, genes involved in formation of the hook-basal body complex belong to the class-2 operons, whereas those involved in formation of filament belong to the class-3 operons. The fliA gene encodes an alternative sigma factor specific for transcription of the class-3 operons. A negative regulatory gene, flgM, which is responsible for the coupling of expression of class-3 operons to flagellar assembly, encodes an anti-sigma factor that binds to FliA and prevents its association with RNA polymerase core enzyme. In the present study, we showed that the flgM gene is transcribed from two different promoters: one is its own class-3 promoter and the other is the class-2 promoter for the upstream gene, flgA. Furthermore, we showed that FlgM is excreted into culture medium from cells of the wild-type strain and of class-3 mutants that can produce complete hook-basal body structures. On the other hand, FlgM is not excreted from mutants defective in the hook-basal body genes. These results indicate that FlgM is excreted from the cells through the flagellar substructures that are formed by the function of the hook-basal body genes.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Kutsukake K
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Japan.

MeSH

Bacterial ProteinsFlagellaGene Expression Regulation, BacterialGenes, BacterialGenes, RegulatorModels, GeneticPromoter Regions, GeneticRNA, MessengerRecombinant Fusion ProteinsSalmonella typhimuriumSigma FactorTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8028576

Citation

Kutsukake, K. "Excretion of the Anti-sigma Factor Through a Flagellar Substructure Couples Flagellar Gene Expression With Flagellar Assembly in Salmonella Typhimurium." Molecular & General Genetics : MGG, vol. 243, no. 6, 1994, pp. 605-12.
Kutsukake K. Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium. Mol Gen Genet. 1994;243(6):605-12.
Kutsukake, K. (1994). Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium. Molecular & General Genetics : MGG, 243(6), 605-12.
Kutsukake K. Excretion of the Anti-sigma Factor Through a Flagellar Substructure Couples Flagellar Gene Expression With Flagellar Assembly in Salmonella Typhimurium. Mol Gen Genet. 1994 Jun 15;243(6):605-12. PubMed PMID: 8028576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium. A1 - Kutsukake,K, PY - 1994/6/15/pubmed PY - 1994/6/15/medline PY - 1994/6/15/entrez SP - 605 EP - 12 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 243 IS - 6 N2 - More than 50 genes are required for flagellar formation and function in Salmonella typhimurium. According to the cascade model of the flagellar regulon, the flagellar operons are divided into three classes, 1, 2, and 3, with reference to their relative positions in the transcriptional hierarchy. This sequential transcription is coupled to the assembly process of the flagellar structure, that is, genes involved in formation of the hook-basal body complex belong to the class-2 operons, whereas those involved in formation of filament belong to the class-3 operons. The fliA gene encodes an alternative sigma factor specific for transcription of the class-3 operons. A negative regulatory gene, flgM, which is responsible for the coupling of expression of class-3 operons to flagellar assembly, encodes an anti-sigma factor that binds to FliA and prevents its association with RNA polymerase core enzyme. In the present study, we showed that the flgM gene is transcribed from two different promoters: one is its own class-3 promoter and the other is the class-2 promoter for the upstream gene, flgA. Furthermore, we showed that FlgM is excreted into culture medium from cells of the wild-type strain and of class-3 mutants that can produce complete hook-basal body structures. On the other hand, FlgM is not excreted from mutants defective in the hook-basal body genes. These results indicate that FlgM is excreted from the cells through the flagellar substructures that are formed by the function of the hook-basal body genes.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/8028576/Excretion_of_the_anti_sigma_factor_through_a_flagellar_substructure_couples_flagellar_gene_expression_with_flagellar_assembly_in_Salmonella_typhimurium_ L2 - https://doi.org/10.1007/BF00279569 DB - PRIME DP - Unbound Medicine ER -