TY - JOUR T1 - Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S. cerevisiae. AU - Chanfreau,G, AU - Legrain,P, AU - Dujon,B, AU - Jacquier,A, PY - 1994/6/11/pubmed PY - 1994/6/11/medline PY - 1994/6/11/entrez SP - 1981 EP - 7 JF - Nucleic acids research JO - Nucleic Acids Res VL - 22 IS - 11 N2 - The splicing of group II and nuclear pre-mRNAs introns occurs via a similar splicing pathway and some of the RNA-RNA interactions involved in these splicing reactions show structural similarities. Recently, genetic analyses performed in a group II intron and the yeast nuclear actin gene suggested that non Watson-Crick interactions between intron boundaries are important for the second splicing step efficiency in both classes of introns. We here show that, in the yeast nuclear rp51A intron, a G to A mutation at the first position activates cryptic 3' splice sites with the sequences UAC/ or UAA/. Moreover, the natural 3' splice site could be reactivated by a G to C substitution of the last intron nucleotide. These results demonstrate that the interaction between the first and last intron nucleotides is a conserved feature of nuclear pre-mRNA splicing in yeast and is involved in the mechanism of 3' splice site selection. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/8029003/Interaction_between_the_first_and_last_nucleotides_of_pre_mRNA_introns_is_a_determinant_of_3'_splice_site_selection_in_S__cerevisiae_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/22.11.1981 DB - PRIME DP - Unbound Medicine ER -