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Effect of glutamine and chemotherapy on protein metabolism in tumor-bearing rats.
J Surg Res. 1994 Jul; 57(1):143-9.JS

Abstract

Supplemental glutamine prevents gut atrophy and enhances muscle protein synthesis in septic rats. This study investigated the effect of glutamine administration and mitomycin C treatment on protein turnover in tumor-bearing rats. AH109A rat ascites hepatoma cells (2 x 10(6)) were subcutaneously implanted in the back of male Donryu rats (n = 32, body weight 150-200 g) on Day 0. The animals were then fed rat chow ad libitum for 10 days. On Day 10, the rats were catheterized for TPN and randomized into four groups according to diet and treatment. The groups were: (i) standard total parenteral nutrition (STPN) + saline; (ii) glutamine-supplemented TPN (GTPN) + saline; (iii) STPN+mitomycin C (MMC); (iv) GTPN+MMC. GTPN was isocaloric (250 kcal/kg/day) and isonitrogenous (1.5 gN/kg/day) with STPN. The animals were maintained on TPN for 5 days and received mitomycin C (0.5 mg/kg) via the catheter every day. On the fifth day of TPN, [1-14C]leucine was given via a 5-hr continuous infusion (2.0 microCi/hr/rat) to determine the fractional synthesis rate of muscle, gut mucosa, liver, and tumor. Also, endogenous leucine production (not equal to whole body protein breakdown rate) was calculated. Body weight loss during TPN was reduced with GTPN. GTPN enhanced muscle FSR in untreated animals (STPN: 10.8 +/- 8.7%/day vs GTPN: 14.7 +/- 0.6%/day, P < 0.05) and in mitomycin C-treated animals (STPN+MMC: 9.6 +/- 0.9%/day, GTPN+MMC: 12.0 +/- 0.8%/day, P < 0.05). The whole body protein breakdown rate was reduced with GTPN. Mitomycin C reduced the mucosal fractional synthesis rate and GTPN did not prevent this reduction.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

1st Department of Surgery, School of Medicine, Kurume University, Fukuoko, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8041129

Citation

Kaibara, A, et al. "Effect of Glutamine and Chemotherapy On Protein Metabolism in Tumor-bearing Rats." The Journal of Surgical Research, vol. 57, no. 1, 1994, pp. 143-9.
Kaibara A, Yoshida S, Yamasaki K, et al. Effect of glutamine and chemotherapy on protein metabolism in tumor-bearing rats. J Surg Res. 1994;57(1):143-9.
Kaibara, A., Yoshida, S., Yamasaki, K., Ishibashi, N., & Kakegawa, T. (1994). Effect of glutamine and chemotherapy on protein metabolism in tumor-bearing rats. The Journal of Surgical Research, 57(1), 143-9.
Kaibara A, et al. Effect of Glutamine and Chemotherapy On Protein Metabolism in Tumor-bearing Rats. J Surg Res. 1994;57(1):143-9. PubMed PMID: 8041129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of glutamine and chemotherapy on protein metabolism in tumor-bearing rats. AU - Kaibara,A, AU - Yoshida,S, AU - Yamasaki,K, AU - Ishibashi,N, AU - Kakegawa,T, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 143 EP - 9 JF - The Journal of surgical research JO - J Surg Res VL - 57 IS - 1 N2 - Supplemental glutamine prevents gut atrophy and enhances muscle protein synthesis in septic rats. This study investigated the effect of glutamine administration and mitomycin C treatment on protein turnover in tumor-bearing rats. AH109A rat ascites hepatoma cells (2 x 10(6)) were subcutaneously implanted in the back of male Donryu rats (n = 32, body weight 150-200 g) on Day 0. The animals were then fed rat chow ad libitum for 10 days. On Day 10, the rats were catheterized for TPN and randomized into four groups according to diet and treatment. The groups were: (i) standard total parenteral nutrition (STPN) + saline; (ii) glutamine-supplemented TPN (GTPN) + saline; (iii) STPN+mitomycin C (MMC); (iv) GTPN+MMC. GTPN was isocaloric (250 kcal/kg/day) and isonitrogenous (1.5 gN/kg/day) with STPN. The animals were maintained on TPN for 5 days and received mitomycin C (0.5 mg/kg) via the catheter every day. On the fifth day of TPN, [1-14C]leucine was given via a 5-hr continuous infusion (2.0 microCi/hr/rat) to determine the fractional synthesis rate of muscle, gut mucosa, liver, and tumor. Also, endogenous leucine production (not equal to whole body protein breakdown rate) was calculated. Body weight loss during TPN was reduced with GTPN. GTPN enhanced muscle FSR in untreated animals (STPN: 10.8 +/- 8.7%/day vs GTPN: 14.7 +/- 0.6%/day, P < 0.05) and in mitomycin C-treated animals (STPN+MMC: 9.6 +/- 0.9%/day, GTPN+MMC: 12.0 +/- 0.8%/day, P < 0.05). The whole body protein breakdown rate was reduced with GTPN. Mitomycin C reduced the mucosal fractional synthesis rate and GTPN did not prevent this reduction.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/8041129/Effect_of_glutamine_and_chemotherapy_on_protein_metabolism_in_tumor_bearing_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(84)71122-X DB - PRIME DP - Unbound Medicine ER -