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Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex.
Gene. 1994 Jul 22; 145(1):9-15.GENE

Abstract

The Escherichia coli otsBA operon, located at min 42, was sequenced and shown to encode a 29.1-kDa trehalose-6-phosphate phosphatase (OtsB) and a 53.6-kDa trehalose-6-phosphate synthase (OtsA). Both proteins display sequence homology with subunits of the Saccharomyces cerevisiae trehalose-6-phosphate synthase/phosphatase complex, which is made up of the subunits TPS1, TPS2 and TPS3 (TSL1). OtsA has homology to the full-length TPS1, the N-terminal part of TPS2 and an internal region of TPS3 (TSL1). OtsB has homology to the C-terminal part of TPS2, but no homology to the other subunits. Primer extension analysis showed only one transcription start point upstream from otsB and one upstream from otsA, regardless of the growth conditions tested. The start codons of the otsB and otsA genes were established by N-terminal sequence determination of the proteins. The 3' end of the otsB coding region overlaps the 5' end of the otsA coding region by 23 nucleotides. The araH gene is located directly upstream from otsBA, and otsB may be identical to pexA.

Authors+Show Affiliations

Norwegian College of Fishery Science, University of Tromsø.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8045430

Citation

Kaasen, I, et al. "Analysis of the otsBA Operon for Osmoregulatory Trehalose Synthesis in Escherichia Coli and Homology of the OtsA and OtsB Proteins to the Yeast Trehalose-6-phosphate Synthase/phosphatase Complex." Gene, vol. 145, no. 1, 1994, pp. 9-15.
Kaasen I, McDougall J, Strøm AR. Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. Gene. 1994;145(1):9-15.
Kaasen, I., McDougall, J., & Strøm, A. R. (1994). Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. Gene, 145(1), 9-15.
Kaasen I, McDougall J, Strøm AR. Analysis of the otsBA Operon for Osmoregulatory Trehalose Synthesis in Escherichia Coli and Homology of the OtsA and OtsB Proteins to the Yeast Trehalose-6-phosphate Synthase/phosphatase Complex. Gene. 1994 Jul 22;145(1):9-15. PubMed PMID: 8045430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. AU - Kaasen,I, AU - McDougall,J, AU - Strøm,A R, PY - 1994/7/22/pubmed PY - 1994/7/22/medline PY - 1994/7/22/entrez SP - 9 EP - 15 JF - Gene JO - Gene VL - 145 IS - 1 N2 - The Escherichia coli otsBA operon, located at min 42, was sequenced and shown to encode a 29.1-kDa trehalose-6-phosphate phosphatase (OtsB) and a 53.6-kDa trehalose-6-phosphate synthase (OtsA). Both proteins display sequence homology with subunits of the Saccharomyces cerevisiae trehalose-6-phosphate synthase/phosphatase complex, which is made up of the subunits TPS1, TPS2 and TPS3 (TSL1). OtsA has homology to the full-length TPS1, the N-terminal part of TPS2 and an internal region of TPS3 (TSL1). OtsB has homology to the C-terminal part of TPS2, but no homology to the other subunits. Primer extension analysis showed only one transcription start point upstream from otsB and one upstream from otsA, regardless of the growth conditions tested. The start codons of the otsB and otsA genes were established by N-terminal sequence determination of the proteins. The 3' end of the otsB coding region overlaps the 5' end of the otsA coding region by 23 nucleotides. The araH gene is located directly upstream from otsBA, and otsB may be identical to pexA. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/8045430/Analysis_of_the_otsBA_operon_for_osmoregulatory_trehalose_synthesis_in_Escherichia_coli_and_homology_of_the_OtsA_and_OtsB_proteins_to_the_yeast_trehalose_6_phosphate_synthase/phosphatase_complex_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(94)90316-6 DB - PRIME DP - Unbound Medicine ER -