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Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium.
Arch Microbiol. 1994; 161(6):501-7.AM

Abstract

The murB gene of Salmonella typhimurium was cloned and found to be 75% and 82% identical to the DNA and protein sequences, respectively, of the same gene in Escherichia coli. These identities are among the lowest recorded between the two bacteria. Nevertheless, wild-type S. typhimurium murB complemented the known temperature-sensitive E. coli mutant, and wild-type E. coli murB complemented three temperature-sensitive mutants of S. typhimurium. The 5S rRNA gene, rrfB, and the region between murB and rrfB were also cloned and sequenced. The rrfB gene of S. typhimurium differs from rrfB of E. coli in only 2 of 120 nt, but the region between murB and rrfB has diverged greatly and includes a sequence that closely resembles a repetitive extragenic palindrome of the type normally associated with E. coli. Previous comparisons of gene divergence have suggested that the chromosomal mutation rate is lower in the vicinity of the origin of replication. However, the S. typhimurium murB gene, located 6 map minutes from the origin of replication, is highly substituted at synonymous sites and the sequence between murB and rrfB is significantly modified as well. Thus, murB is an exception to the general observation that genes near the origin of replication show less divergence than do genes elsewhere in the bacterial chromosome.

Authors+Show Affiliations

Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks 58202-9001.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8048842

Citation

Dombrosky, P M., et al. "Sequence Divergence of the murB and rrfB Genes From Escherichia Coli and Salmonella Typhimurium." Archives of Microbiology, vol. 161, no. 6, 1994, pp. 501-7.
Dombrosky PM, Schmid MB, Young KD. Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium. Arch Microbiol. 1994;161(6):501-7.
Dombrosky, P. M., Schmid, M. B., & Young, K. D. (1994). Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium. Archives of Microbiology, 161(6), 501-7.
Dombrosky PM, Schmid MB, Young KD. Sequence Divergence of the murB and rrfB Genes From Escherichia Coli and Salmonella Typhimurium. Arch Microbiol. 1994;161(6):501-7. PubMed PMID: 8048842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium. AU - Dombrosky,P M, AU - Schmid,M B, AU - Young,K D, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 501 EP - 7 JF - Archives of microbiology JO - Arch Microbiol VL - 161 IS - 6 N2 - The murB gene of Salmonella typhimurium was cloned and found to be 75% and 82% identical to the DNA and protein sequences, respectively, of the same gene in Escherichia coli. These identities are among the lowest recorded between the two bacteria. Nevertheless, wild-type S. typhimurium murB complemented the known temperature-sensitive E. coli mutant, and wild-type E. coli murB complemented three temperature-sensitive mutants of S. typhimurium. The 5S rRNA gene, rrfB, and the region between murB and rrfB were also cloned and sequenced. The rrfB gene of S. typhimurium differs from rrfB of E. coli in only 2 of 120 nt, but the region between murB and rrfB has diverged greatly and includes a sequence that closely resembles a repetitive extragenic palindrome of the type normally associated with E. coli. Previous comparisons of gene divergence have suggested that the chromosomal mutation rate is lower in the vicinity of the origin of replication. However, the S. typhimurium murB gene, located 6 map minutes from the origin of replication, is highly substituted at synonymous sites and the sequence between murB and rrfB is significantly modified as well. Thus, murB is an exception to the general observation that genes near the origin of replication show less divergence than do genes elsewhere in the bacterial chromosome. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/8048842/Sequence_divergence_of_the_murB_and_rrfB_genes_from_Escherichia_coli_and_Salmonella_typhimurium_ DB - PRIME DP - Unbound Medicine ER -