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Splicing of the alternative exons of the chicken, rat, and Xenopus beta tropomyosin transcripts requires class-specific elements.
J Biol Chem. 1994 Aug 05; 269(31):19675-8.JB

Abstract

The diversity of protein isoforms is often generated from single genes by alternative splicing of the primary transcript. Using transfection of beta tropomyosin minigene constructs into homologous and heterologous cell systems, we show that there are differences, among higher vertebrates, in the components of the splicing machinery which control the conserved regulated splicing pattern of two mutually exclusive exons (6A and 6B) present in this gene. These experiments demonstrate that genes which give rise to alternative transcripts may require an appropriate combination of splicing factors which are species-specific, or at least restricted to the same taxonomic subgroup (class). An important practical implication is that the splicing of these genes may be deregulated in heterologous systems in vitro and in vivo, i.e. in transgenic animals.

Authors+Show Affiliations

Département de Biologie Moléculaire, Institut Pasteur, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8051042

Citation

Balvay, L, et al. "Splicing of the Alternative Exons of the Chicken, Rat, and Xenopus Beta Tropomyosin Transcripts Requires Class-specific Elements." The Journal of Biological Chemistry, vol. 269, no. 31, 1994, pp. 19675-8.
Balvay L, Pret AM, Libri D, et al. Splicing of the alternative exons of the chicken, rat, and Xenopus beta tropomyosin transcripts requires class-specific elements. J Biol Chem. 1994;269(31):19675-8.
Balvay, L., Pret, A. M., Libri, D., Helfman, D. M., & Fiszman, M. Y. (1994). Splicing of the alternative exons of the chicken, rat, and Xenopus beta tropomyosin transcripts requires class-specific elements. The Journal of Biological Chemistry, 269(31), 19675-8.
Balvay L, et al. Splicing of the Alternative Exons of the Chicken, Rat, and Xenopus Beta Tropomyosin Transcripts Requires Class-specific Elements. J Biol Chem. 1994 Aug 5;269(31):19675-8. PubMed PMID: 8051042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Splicing of the alternative exons of the chicken, rat, and Xenopus beta tropomyosin transcripts requires class-specific elements. AU - Balvay,L, AU - Pret,A M, AU - Libri,D, AU - Helfman,D M, AU - Fiszman,M Y, PY - 1994/8/5/pubmed PY - 1994/8/5/medline PY - 1994/8/5/entrez SP - 19675 EP - 8 JF - The Journal of biological chemistry JO - J Biol Chem VL - 269 IS - 31 N2 - The diversity of protein isoforms is often generated from single genes by alternative splicing of the primary transcript. Using transfection of beta tropomyosin minigene constructs into homologous and heterologous cell systems, we show that there are differences, among higher vertebrates, in the components of the splicing machinery which control the conserved regulated splicing pattern of two mutually exclusive exons (6A and 6B) present in this gene. These experiments demonstrate that genes which give rise to alternative transcripts may require an appropriate combination of splicing factors which are species-specific, or at least restricted to the same taxonomic subgroup (class). An important practical implication is that the splicing of these genes may be deregulated in heterologous systems in vitro and in vivo, i.e. in transgenic animals. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/8051042/Splicing_of_the_alternative_exons_of_the_chicken_rat_and_Xenopus_beta_tropomyosin_transcripts_requires_class_specific_elements_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(17)32071-9 DB - PRIME DP - Unbound Medicine ER -