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Crystallization and preliminary X-ray diffraction studies on the Apo form of orotate phosphoribosyltransferase from Escherichia coli.
J Mol Biol. 1994 Aug 12; 241(2):292-4.JM

Abstract

Three different crystal forms of the apoenzyme orotate phosphoribosyltransferase, with M(r) = 23,552 from Escherichia coli have been grown. The crystals, which are all suitable for X-ray diffraction analysis, have been grown by the hanging drop vapour diffusion method. The first form crystallizes in the orthorhombic space group P2(1)2(1)2, with cell dimensions: a = 136.34 A, b = 75.98 A and c = 40.32 A; the second form in the monoclinic space group P2, with unit cell dimensions: a = 101.61 A, b = 40.49 A, c = 79.05 A and beta = 87.33 degrees; and the third form in P2(1)2(1)2(1), the cell dimensions being a = 70.27 A, b = 103.53 A, c = 53.83 A.

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Authors+Show Affiliations

Aghajari N
Center for Crystallographic Studies, University of Copenhagen, Denmark.
Jensen KF
No affiliation info available
Gajhede M
No affiliation info available

MeSH

CrystallizationCrystallography, X-RayEscherichia coliMolecular WeightOrotate Phosphoribosyltransferase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8057372

Citation

Aghajari, N, et al. "Crystallization and Preliminary X-ray Diffraction Studies On the Apo Form of Orotate Phosphoribosyltransferase From Escherichia Coli." Journal of Molecular Biology, vol. 241, no. 2, 1994, pp. 292-4.
Aghajari N, Jensen KF, Gajhede M. Crystallization and preliminary X-ray diffraction studies on the Apo form of orotate phosphoribosyltransferase from Escherichia coli. J Mol Biol. 1994;241(2):292-4.
Aghajari, N., Jensen, K. F., & Gajhede, M. (1994). Crystallization and preliminary X-ray diffraction studies on the Apo form of orotate phosphoribosyltransferase from Escherichia coli. Journal of Molecular Biology, 241(2), 292-4.
Aghajari N, Jensen KF, Gajhede M. Crystallization and Preliminary X-ray Diffraction Studies On the Apo Form of Orotate Phosphoribosyltransferase From Escherichia Coli. J Mol Biol. 1994 Aug 12;241(2):292-4. PubMed PMID: 8057372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystallization and preliminary X-ray diffraction studies on the Apo form of orotate phosphoribosyltransferase from Escherichia coli. AU - Aghajari,N, AU - Jensen,K F, AU - Gajhede,M, PY - 1994/8/12/pubmed PY - 1994/8/12/medline PY - 1994/8/12/entrez SP - 292 EP - 4 JF - Journal of molecular biology JO - J Mol Biol VL - 241 IS - 2 N2 - Three different crystal forms of the apoenzyme orotate phosphoribosyltransferase, with M(r) = 23,552 from Escherichia coli have been grown. The crystals, which are all suitable for X-ray diffraction analysis, have been grown by the hanging drop vapour diffusion method. The first form crystallizes in the orthorhombic space group P2(1)2(1)2, with cell dimensions: a = 136.34 A, b = 75.98 A and c = 40.32 A; the second form in the monoclinic space group P2, with unit cell dimensions: a = 101.61 A, b = 40.49 A, c = 79.05 A and beta = 87.33 degrees; and the third form in P2(1)2(1)2(1), the cell dimensions being a = 70.27 A, b = 103.53 A, c = 53.83 A. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/8057372/Crystallization_and_preliminary_X_ray_diffraction_studies_on_the_Apo_form_of_orotate_phosphoribosyltransferase_from_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(84)71503-8 DB - PRIME DP - Unbound Medicine ER -