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[Detection of anti-gonococcal antibodies using a dot immunobinding assay in a high risk female population].
Rev Med Chil. 1994 Jan; 122(1):42-7.RM

Abstract

The bacteriological diagnosis of gonorrhea in women has a low efficiency. A dot immunobinding assay, using whole piliated Neisseria gonorrhoeae cells, was used to detect antigonococcal antibodies in 300 prostitutes in whom a parallel Thayer-Martin culture of endocervical secretion was performed. Twenty sera from men with bacteriologically confirmed gonorrhoea were used as positive controls and 20 sera from children without history of gonococcal infections, as negative controls. Antibodies were detected in 49.7% of women and culture was positive in only one. All men with gonorrhea has positive titers and all sera from children were negative, except one that had a cross-reaction. Test sensitivity in the female population was 1 and specificity 0.5. There were no false negative reactions. Although the test detected antibodies in high risk women, there were cross-reactions. Therefore, the technique should be improved, using a more specific antigen.

Authors+Show Affiliations

Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

8066342

Citation

Soto, L, et al. "[Detection of Anti-gonococcal Antibodies Using a Dot Immunobinding Assay in a High Risk Female Population]." Revista Medica De Chile, vol. 122, no. 1, 1994, pp. 42-7.
Soto L, Rodríguez G, Iglesias T, et al. [Detection of anti-gonococcal antibodies using a dot immunobinding assay in a high risk female population]. Rev Med Chil. 1994;122(1):42-7.
Soto, L., Rodríguez, G., Iglesias, T., Loyola, A., Boehme, C., Polette, M., & Díaz, M. (1994). [Detection of anti-gonococcal antibodies using a dot immunobinding assay in a high risk female population]. Revista Medica De Chile, 122(1), 42-7.
Soto L, et al. [Detection of Anti-gonococcal Antibodies Using a Dot Immunobinding Assay in a High Risk Female Population]. Rev Med Chil. 1994;122(1):42-7. PubMed PMID: 8066342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of anti-gonococcal antibodies using a dot immunobinding assay in a high risk female population]. AU - Soto,L, AU - Rodríguez,G, AU - Iglesias,T, AU - Loyola,A, AU - Boehme,C, AU - Polette,M, AU - Díaz,M, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 42 EP - 7 JF - Revista medica de Chile JO - Rev Med Chil VL - 122 IS - 1 N2 - The bacteriological diagnosis of gonorrhea in women has a low efficiency. A dot immunobinding assay, using whole piliated Neisseria gonorrhoeae cells, was used to detect antigonococcal antibodies in 300 prostitutes in whom a parallel Thayer-Martin culture of endocervical secretion was performed. Twenty sera from men with bacteriologically confirmed gonorrhoea were used as positive controls and 20 sera from children without history of gonococcal infections, as negative controls. Antibodies were detected in 49.7% of women and culture was positive in only one. All men with gonorrhea has positive titers and all sera from children were negative, except one that had a cross-reaction. Test sensitivity in the female population was 1 and specificity 0.5. There were no false negative reactions. Although the test detected antibodies in high risk women, there were cross-reactions. Therefore, the technique should be improved, using a more specific antigen. SN - 0034-9887 UR - https://www.unboundmedicine.com/medline/citation/8066342/[Detection_of_anti_gonococcal_antibodies_using_a_dot_immunobinding_assay_in_a_high_risk_female_population]_ L2 - https://medlineplus.gov/gonorrhea.html DB - PRIME DP - Unbound Medicine ER -