Tags

Type your tag names separated by a space and hit enter

Restoration of cyclophosphamide-suppressed primary immune response in mice by Tołpa Peat Preparation (TPP)--comparison with calf thymus extract.
Acta Pol Pharm 1993; 50(6):501-6AP

Abstract

In order to induce pharmacological immunosuppression in Balb/c mice, the animals were given cyclophosphamide intraperitoneally, 200 mg/kg 144 hours or 24 hours prior to SRBC immunization. Tołpa Peat Preparation (TPP) was administered in doses of 10 and 50 mg/kg on the day of immunization and then every 24 hours for 3 consecutive days. It was given both to mice cyclophosphamide-treated and non-treated. Calf thymus extract (TFX) was administered (1 and 5 mg/kg) parallelly, for comparison with TPP. The following immunologic indices were determined: percent of splenocytes forming E- and EAC-rosettes, number of plaque-forming cells (PFC) and the levels of 19S+7S and 7S anti-SRBC haemagglutinins. The immunosuppressing action of a single cyclophosphamide dose decreased the percentage of EAC rosettes, number of PFC and serum anti-SRBC haemagglutinin titre. TPP is able to restore the percent of EAC rosettes, the number of PFC and anti-SRBC haemagglutinin titre, either partially or totally, after cyclophosphamide-induced immunosuppression. The effectiveness of TPP depends mainly on the dose and timing of cyclophosphamide administration prior to SRBC immunization. The most pronounced restoration of the cells in mice was observed with the TPP dose of 50 mg/kg after cyclophosphamide suppression 144 h before immunization. TFX (1 mg/kg) only partially restored the number of PFC and 7S anti-SRBC haemagglutinin titre, which had been depressed due to cyclophosphamide treatment 144 h prior to immunization. However, it did not change the depressing effect of cyclophosphamide on the percent of EAC rosette-forming splenocytes.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agriculture University, Wrocław, Poland.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8067263

Citation

Obmińska-Domoradzka, B. "Restoration of Cyclophosphamide-suppressed Primary Immune Response in Mice By Tołpa Peat Preparation (TPP)--comparison With Calf Thymus Extract." Acta Poloniae Pharmaceutica, vol. 50, no. 6, 1993, pp. 501-6.
Obmińska-Domoradzka B. Restoration of cyclophosphamide-suppressed primary immune response in mice by Tołpa Peat Preparation (TPP)--comparison with calf thymus extract. Acta Pol Pharm. 1993;50(6):501-6.
Obmińska-Domoradzka, B. (1993). Restoration of cyclophosphamide-suppressed primary immune response in mice by Tołpa Peat Preparation (TPP)--comparison with calf thymus extract. Acta Poloniae Pharmaceutica, 50(6), pp. 501-6.
Obmińska-Domoradzka B. Restoration of Cyclophosphamide-suppressed Primary Immune Response in Mice By Tołpa Peat Preparation (TPP)--comparison With Calf Thymus Extract. Acta Pol Pharm. 1993;50(6):501-6. PubMed PMID: 8067263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Restoration of cyclophosphamide-suppressed primary immune response in mice by Tołpa Peat Preparation (TPP)--comparison with calf thymus extract. A1 - Obmińska-Domoradzka,B, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 501 EP - 6 JF - Acta poloniae pharmaceutica JO - Acta Pol Pharm VL - 50 IS - 6 N2 - In order to induce pharmacological immunosuppression in Balb/c mice, the animals were given cyclophosphamide intraperitoneally, 200 mg/kg 144 hours or 24 hours prior to SRBC immunization. Tołpa Peat Preparation (TPP) was administered in doses of 10 and 50 mg/kg on the day of immunization and then every 24 hours for 3 consecutive days. It was given both to mice cyclophosphamide-treated and non-treated. Calf thymus extract (TFX) was administered (1 and 5 mg/kg) parallelly, for comparison with TPP. The following immunologic indices were determined: percent of splenocytes forming E- and EAC-rosettes, number of plaque-forming cells (PFC) and the levels of 19S+7S and 7S anti-SRBC haemagglutinins. The immunosuppressing action of a single cyclophosphamide dose decreased the percentage of EAC rosettes, number of PFC and serum anti-SRBC haemagglutinin titre. TPP is able to restore the percent of EAC rosettes, the number of PFC and anti-SRBC haemagglutinin titre, either partially or totally, after cyclophosphamide-induced immunosuppression. The effectiveness of TPP depends mainly on the dose and timing of cyclophosphamide administration prior to SRBC immunization. The most pronounced restoration of the cells in mice was observed with the TPP dose of 50 mg/kg after cyclophosphamide suppression 144 h before immunization. TFX (1 mg/kg) only partially restored the number of PFC and 7S anti-SRBC haemagglutinin titre, which had been depressed due to cyclophosphamide treatment 144 h prior to immunization. However, it did not change the depressing effect of cyclophosphamide on the percent of EAC rosette-forming splenocytes.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0001-6837 UR - https://www.unboundmedicine.com/medline/citation/8067263/Restoration_of_cyclophosphamide_suppressed_primary_immune_response_in_mice_by_Tołpa_Peat_Preparation__TPP___comparison_with_calf_thymus_extract_ DB - PRIME DP - Unbound Medicine ER -