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Effect of L-carnitine on mitochondrial acyl CoA esters in the ischemic dog heart.
J Mol Cell Cardiol. 1994 Apr; 26(4):499-508.JM

Abstract

Many studies have shown that L-carnitine has a positive effect on ischemic myocardium, probably by reducing accumulation of long-chain acyl coenzyme A (CoA) esters. Previous studies have involved whole-heart extracts and have not assessed changes of CoA ester levels in mitochondria, the site of translocase inhibition. To more precisely assess L-carnitine effects, we measured long-chain acyl CoA ester levels in cytosol and in mitochondria in the ischemic canine heart. Dogs were divided into four groups: a sham-operated control group; an untreated group; and high- and low-dose L-carnitine-treated groups (30 mg/kg and 100 mg/kg). After 60 min of ischemia, the heart was excised, and the cytosolic and mitochondrial fractions were isolated. CoA esters and the activity of carnitine palmitoylcarnitine transferase (CPT) I and II were measured in both compartments. Approximately 89% of cellular free CoA. 90% of cellular acetyl CoA, 97% of cellular shot-chain acyl CoA, and 92% of cellular long-chain acyl CoA were located in the mitochondrial space under the normal condition. Under the ischemic condition, mitochondrial free CoA was significantly decreased. Conversely, mitochondrial acetyl CoA and long-chain acyl CoA were significantly increased. Treatment with L-carnitine significantly decreased acetyl CoA and long-chain acyl CoA in the ischemic mitochondrial space in a dose-dependent manner. These results support the hypothesis that L-carnitine reduces accumulation of long-chain acyl CoA within the ischemic mitochondrial space and thereby improves mitochondrial function and adenine nucleotide translocation.

Authors+Show Affiliations

Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8072006

Citation

Kobayashi, A, and S Fujisawa. "Effect of L-carnitine On Mitochondrial Acyl CoA Esters in the Ischemic Dog Heart." Journal of Molecular and Cellular Cardiology, vol. 26, no. 4, 1994, pp. 499-508.
Kobayashi A, Fujisawa S. Effect of L-carnitine on mitochondrial acyl CoA esters in the ischemic dog heart. J Mol Cell Cardiol. 1994;26(4):499-508.
Kobayashi, A., & Fujisawa, S. (1994). Effect of L-carnitine on mitochondrial acyl CoA esters in the ischemic dog heart. Journal of Molecular and Cellular Cardiology, 26(4), 499-508.
Kobayashi A, Fujisawa S. Effect of L-carnitine On Mitochondrial Acyl CoA Esters in the Ischemic Dog Heart. J Mol Cell Cardiol. 1994;26(4):499-508. PubMed PMID: 8072006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of L-carnitine on mitochondrial acyl CoA esters in the ischemic dog heart. AU - Kobayashi,A, AU - Fujisawa,S, PY - 1994/4/1/pubmed PY - 1994/4/1/medline PY - 1994/4/1/entrez SP - 499 EP - 508 JF - Journal of molecular and cellular cardiology JO - J Mol Cell Cardiol VL - 26 IS - 4 N2 - Many studies have shown that L-carnitine has a positive effect on ischemic myocardium, probably by reducing accumulation of long-chain acyl coenzyme A (CoA) esters. Previous studies have involved whole-heart extracts and have not assessed changes of CoA ester levels in mitochondria, the site of translocase inhibition. To more precisely assess L-carnitine effects, we measured long-chain acyl CoA ester levels in cytosol and in mitochondria in the ischemic canine heart. Dogs were divided into four groups: a sham-operated control group; an untreated group; and high- and low-dose L-carnitine-treated groups (30 mg/kg and 100 mg/kg). After 60 min of ischemia, the heart was excised, and the cytosolic and mitochondrial fractions were isolated. CoA esters and the activity of carnitine palmitoylcarnitine transferase (CPT) I and II were measured in both compartments. Approximately 89% of cellular free CoA. 90% of cellular acetyl CoA, 97% of cellular shot-chain acyl CoA, and 92% of cellular long-chain acyl CoA were located in the mitochondrial space under the normal condition. Under the ischemic condition, mitochondrial free CoA was significantly decreased. Conversely, mitochondrial acetyl CoA and long-chain acyl CoA were significantly increased. Treatment with L-carnitine significantly decreased acetyl CoA and long-chain acyl CoA in the ischemic mitochondrial space in a dose-dependent manner. These results support the hypothesis that L-carnitine reduces accumulation of long-chain acyl CoA within the ischemic mitochondrial space and thereby improves mitochondrial function and adenine nucleotide translocation. SN - 0022-2828 UR - https://www.unboundmedicine.com/medline/citation/8072006/Effect_of_L_carnitine_on_mitochondrial_acyl_CoA_esters_in_the_ischemic_dog_heart_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2828(84)71060-1 DB - PRIME DP - Unbound Medicine ER -