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Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71.
Appl Environ Microbiol. 1994 Jul; 60(7):2545-52.AE

Abstract

Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, transcript assays, and expression of enzymatic activities in Escherichia coli have now confirmed that a 3,500-bp segment contains the open reading frames (ORFs) for Pel-3 and Peh-1. The 1,041-bp pel-3 ORF and the 1,206-bp peh-1 ORF are separated by a 579-bp sequence. The genes are transcribed divergently from their own promoters. In E. coli and E. carotovora subsp. carotovora 71, peh-1 is better expressed than pel-3. However, plant signals activate the expression of both the genes in E. carotovora subsp. carotovora. A consensus integration host factor (IHF)-binding sequence upstream of pel-3 appears physiologically significant, since pel-3 promoter activity is higher in an E. coli IHF+ strain than in an IHF- strain. While peh-1 has extensive homology with plant and bacterial peh genes, pel-3 appears not to have significant homology with the pel genes belonging to the pelBC, pelADE, or periplasmic pel families. Pel-3 also is unusual in that it is predicted to contain an ATP- and GTP-binding site motif A (P-loop) not found in the other Pels.

Authors+Show Affiliations

Department of Plant Pathology, University of Missouri, Columbia 65211.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

8074530

Citation

Liu, Y, et al. "Nucleotide Sequence and Expression of a Novel Pectate Lyase Gene (pel-3) and a Closely Linked Endopolygalacturonase Gene (peh-1) of Erwinia Carotovora Subsp. Carotovora 71." Applied and Environmental Microbiology, vol. 60, no. 7, 1994, pp. 2545-52.
Liu Y, Chatterjee A, Chatterjee AK. Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71. Appl Environ Microbiol. 1994;60(7):2545-52.
Liu, Y., Chatterjee, A., & Chatterjee, A. K. (1994). Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71. Applied and Environmental Microbiology, 60(7), 2545-52.
Liu Y, Chatterjee A, Chatterjee AK. Nucleotide Sequence and Expression of a Novel Pectate Lyase Gene (pel-3) and a Closely Linked Endopolygalacturonase Gene (peh-1) of Erwinia Carotovora Subsp. Carotovora 71. Appl Environ Microbiol. 1994;60(7):2545-52. PubMed PMID: 8074530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71. AU - Liu,Y, AU - Chatterjee,A, AU - Chatterjee,A K, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 2545 EP - 52 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 60 IS - 7 N2 - Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, transcript assays, and expression of enzymatic activities in Escherichia coli have now confirmed that a 3,500-bp segment contains the open reading frames (ORFs) for Pel-3 and Peh-1. The 1,041-bp pel-3 ORF and the 1,206-bp peh-1 ORF are separated by a 579-bp sequence. The genes are transcribed divergently from their own promoters. In E. coli and E. carotovora subsp. carotovora 71, peh-1 is better expressed than pel-3. However, plant signals activate the expression of both the genes in E. carotovora subsp. carotovora. A consensus integration host factor (IHF)-binding sequence upstream of pel-3 appears physiologically significant, since pel-3 promoter activity is higher in an E. coli IHF+ strain than in an IHF- strain. While peh-1 has extensive homology with plant and bacterial peh genes, pel-3 appears not to have significant homology with the pel genes belonging to the pelBC, pelADE, or periplasmic pel families. Pel-3 also is unusual in that it is predicted to contain an ATP- and GTP-binding site motif A (P-loop) not found in the other Pels. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/8074530/Nucleotide_sequence_and_expression_of_a_novel_pectate_lyase_gene__pel_3__and_a_closely_linked_endopolygalacturonase_gene__peh_1__of_Erwinia_carotovora_subsp__carotovora_71_ L2 - https://journals.asm.org/doi/10.1128/aem.60.7.2545-2552.1994?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -