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Expression of transcripts for CSF-1 and for the "macrophage" and "epithelial" isoforms of the CSF-1R transcripts in human ovarian carcinomas.
Cancer Detect Prev. 1994; 18(3):231-9.CD

Abstract

Levels of CSF-1 and CSF-1 receptor (CSF-1R) (fms) transcripts were quantified by Northern and dot blotting in total RNA extracts from 68 human ovarian carcinomas and positive and negative controls. Overall, very high levels of CSF-1 and CSF-1R transcripts were found in 11 and 21% of ovarian carcinoma specimens, respectively. When probes specific for CSF-1R exon1 were employed, which hybridize with the "epithelial" but not with the "hematopoietic" isoforms of the CSF-1R transcripts, the results suggested that both isoforms of the CSF-1R transcript were present in the tumor specimens. When levels of CSF-1 and CSF-1R were compared, a positive correlation was observed. Taken together, these results confirm prior observations of high levels of CSF-1 and CSF-1R in ovarian carcinoma specimens and that the latter include both "epithelial" and "macrophage" CSF-1R transcript isoforms, as might be expected for specimens composed of malignant epithelial cells and stromal macrophages.

Authors+Show Affiliations

Bauknecht T
Department of Gynecology and Obstetrics, Albert-Ludwigs University, Freiburg, Germany.
Kiechle-Schwarz M
No affiliation info available
du Bois A
No affiliation info available
Wölfle J
No affiliation info available
Kacinski B
No affiliation info available

MeSH

CarcinomaEpitheliumFemaleHumansImmunoblottingMacrophage Colony-Stimulating FactorMacrophagesOvarian NeoplasmsRNA, NeoplasmReceptor, Macrophage Colony-Stimulating Factor

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8076385

Citation

Bauknecht, T, et al. "Expression of Transcripts for CSF-1 and for the "macrophage" and "epithelial" Isoforms of the CSF-1R Transcripts in Human Ovarian Carcinomas." Cancer Detection and Prevention, vol. 18, no. 3, 1994, pp. 231-9.
Bauknecht T, Kiechle-Schwarz M, du Bois A, et al. Expression of transcripts for CSF-1 and for the "macrophage" and "epithelial" isoforms of the CSF-1R transcripts in human ovarian carcinomas. Cancer Detect Prev. 1994;18(3):231-9.
Bauknecht, T., Kiechle-Schwarz, M., du Bois, A., Wölfle, J., & Kacinski, B. (1994). Expression of transcripts for CSF-1 and for the "macrophage" and "epithelial" isoforms of the CSF-1R transcripts in human ovarian carcinomas. Cancer Detection and Prevention, 18(3), 231-9.
Bauknecht T, et al. Expression of Transcripts for CSF-1 and for the "macrophage" and "epithelial" Isoforms of the CSF-1R Transcripts in Human Ovarian Carcinomas. Cancer Detect Prev. 1994;18(3):231-9. PubMed PMID: 8076385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of transcripts for CSF-1 and for the "macrophage" and "epithelial" isoforms of the CSF-1R transcripts in human ovarian carcinomas. AU - Bauknecht,T, AU - Kiechle-Schwarz,M, AU - du Bois,A, AU - Wölfle,J, AU - Kacinski,B, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 231 EP - 9 JF - Cancer detection and prevention JO - Cancer Detect Prev VL - 18 IS - 3 N2 - Levels of CSF-1 and CSF-1 receptor (CSF-1R) (fms) transcripts were quantified by Northern and dot blotting in total RNA extracts from 68 human ovarian carcinomas and positive and negative controls. Overall, very high levels of CSF-1 and CSF-1R transcripts were found in 11 and 21% of ovarian carcinoma specimens, respectively. When probes specific for CSF-1R exon1 were employed, which hybridize with the "epithelial" but not with the "hematopoietic" isoforms of the CSF-1R transcripts, the results suggested that both isoforms of the CSF-1R transcript were present in the tumor specimens. When levels of CSF-1 and CSF-1R were compared, a positive correlation was observed. Taken together, these results confirm prior observations of high levels of CSF-1 and CSF-1R in ovarian carcinoma specimens and that the latter include both "epithelial" and "macrophage" CSF-1R transcript isoforms, as might be expected for specimens composed of malignant epithelial cells and stromal macrophages. SN - 0361-090X UR - https://www.unboundmedicine.com/medline/citation/8076385/Expression_of_transcripts_for_CSF_1_and_for_the_"macrophage"_and_"epithelial"_isoforms_of_the_CSF_1R_transcripts_in_human_ovarian_carcinomas_ L2 - https://medlineplus.gov/ovariancancer.html DB - PRIME DP - Unbound Medicine ER -