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Optimization of an HP Scanjet for quantification of protein electrophoresis gels.
Anal Biochem. 1994 Jun; 219(2):297-304.AB

Abstract

An inexpensive desktop scanner, the Hewlett-Packard Scanjet IIp (HP), has been optimized for analysis of protein electrophoresis gels by comparison with a calibrated laser densitometer (Laser). Images from both densitometers were transferred to a personal computer and analyzed with QGEL software. Without correction the HP response was often in poor agreement with the Laser. However, when the HP response to Coomassie blue stained gels and x-ray films was linearized using a HP software option called Emphasis, the HP results agreed with results from the Laser. For 2D gels scanned with appropriate Emphasis applied, spot integrated density values were a constant multiple of 1.8 +/- 0.3 times the corresponding Laser value for x-ray films (CV = 17%) and 2.1 +/- 0.5 for Coomassie blue stained gels (CV = 24%). The highest error was observed for density extremes. For proteins quantified relative to standards using sodium dodecyl sulfate-slab gel electrophoresis, the HP values were within 15% of the Laser values. Data is shown concerning linearity and reproducibility of response, optical density range (about 0 to 1.8 OD units), variability of the imaging field, and resolution of the HP.

Authors+Show Affiliations

Kendrick Laboratories, Inc., Madison, Wisconsin 53713.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8080086

Citation

Kendrick, N C., et al. "Optimization of an HP Scanjet for Quantification of Protein Electrophoresis Gels." Analytical Biochemistry, vol. 219, no. 2, 1994, pp. 297-304.
Kendrick NC, Johansen JJ, Lee PR, et al. Optimization of an HP Scanjet for quantification of protein electrophoresis gels. Anal Biochem. 1994;219(2):297-304.
Kendrick, N. C., Johansen, J. J., Lee, P. R., & Santek, D. A. (1994). Optimization of an HP Scanjet for quantification of protein electrophoresis gels. Analytical Biochemistry, 219(2), 297-304.
Kendrick NC, et al. Optimization of an HP Scanjet for Quantification of Protein Electrophoresis Gels. Anal Biochem. 1994;219(2):297-304. PubMed PMID: 8080086.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of an HP Scanjet for quantification of protein electrophoresis gels. AU - Kendrick,N C, AU - Johansen,J J, AU - Lee,P R, AU - Santek,D A, PY - 1994/6/1/pubmed PY - 1994/6/1/medline PY - 1994/6/1/entrez SP - 297 EP - 304 JF - Analytical biochemistry JO - Anal Biochem VL - 219 IS - 2 N2 - An inexpensive desktop scanner, the Hewlett-Packard Scanjet IIp (HP), has been optimized for analysis of protein electrophoresis gels by comparison with a calibrated laser densitometer (Laser). Images from both densitometers were transferred to a personal computer and analyzed with QGEL software. Without correction the HP response was often in poor agreement with the Laser. However, when the HP response to Coomassie blue stained gels and x-ray films was linearized using a HP software option called Emphasis, the HP results agreed with results from the Laser. For 2D gels scanned with appropriate Emphasis applied, spot integrated density values were a constant multiple of 1.8 +/- 0.3 times the corresponding Laser value for x-ray films (CV = 17%) and 2.1 +/- 0.5 for Coomassie blue stained gels (CV = 24%). The highest error was observed for density extremes. For proteins quantified relative to standards using sodium dodecyl sulfate-slab gel electrophoresis, the HP values were within 15% of the Laser values. Data is shown concerning linearity and reproducibility of response, optical density range (about 0 to 1.8 OD units), variability of the imaging field, and resolution of the HP. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/8080086/Optimization_of_an_HP_Scanjet_for_quantification_of_protein_electrophoresis_gels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(84)71269-3 DB - PRIME DP - Unbound Medicine ER -