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Exogenous IL-7 promotes the growth of CD3-CD4-CD8-CD44+CD25+/- precursor cells and blocks the differentiation pathway of TCR-alpha beta cells in fetal thymus organ culture.
J Immunol. 1993 Apr 01; 150(7):2706-16.JI

Abstract

Addition of human rIL-7 to fetal thymic organ culture started at day 13, 14, or 15 did not influence the number of cells generated during a 12-day culture period. However, the IL-7 treatment resulted in a preferential expansion of cells with a phenotype characteristic for cells at an early step of differentiation. The cells were CD4-CD8-CD3-CD2- and SCA-1+. Analysis of the coordinate expression of CD44 and CD25 on these cells showed that the majority of the cells were either CD44+CD25- or CD44+CD25intermediate. TCR-alpha beta cells were present but in a significantly lower number as compared to the control cultures. The cell number of TCR-gamma delta cells was increased. All these effects were moderate after 6 days, but unequivocal after 12 days of culture. Treatment of the fetal organ culture with mAb-neutralizing murine IL-7 resulted in an inhibition of the proliferation of the fetal thymocytes. No particular subset studied was preferentially inhibited. By using a model of reconstitution of 14-day embryonic thymuses depleted of thymocytes by deoxyguanosine and reconstituted with fetal day 13 liver cells and set up in organ culture with or without IL-7, it was shown in a clear cut way that IL-7 indeed promotes expansion of the early precursor cells and TCR-gamma delta cells, but prevents the generation of TCR-alpha beta cells. In addition, reconstitution experiments were set up in the presence of mAb-neutralizing murine IL-7. This treatment resulted in the inhibition of the growth of the fetal thymocytes without inhibiting preferentially a particular subset. These data indicate that IL-7 acts at an early step of T cell differentiation and plays a role to expand precursor cells, but prevents this population from additional differentiation towards the TCR-alpha beta pathways, whereas the differentiation towards TCR-gamma delta cells is not influenced or even enhanced.

Authors+Show Affiliations

Department of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8095954

Citation

Plum, J, et al. "Exogenous IL-7 Promotes the Growth of CD3-CD4-CD8-CD44+CD25+/- Precursor Cells and Blocks the Differentiation Pathway of TCR-alpha Beta Cells in Fetal Thymus Organ Culture." Journal of Immunology (Baltimore, Md. : 1950), vol. 150, no. 7, 1993, pp. 2706-16.
Plum J, De Smedt M, Leclercq G. Exogenous IL-7 promotes the growth of CD3-CD4-CD8-CD44+CD25+/- precursor cells and blocks the differentiation pathway of TCR-alpha beta cells in fetal thymus organ culture. J Immunol. 1993;150(7):2706-16.
Plum, J., De Smedt, M., & Leclercq, G. (1993). Exogenous IL-7 promotes the growth of CD3-CD4-CD8-CD44+CD25+/- precursor cells and blocks the differentiation pathway of TCR-alpha beta cells in fetal thymus organ culture. Journal of Immunology (Baltimore, Md. : 1950), 150(7), 2706-16.
Plum J, De Smedt M, Leclercq G. Exogenous IL-7 Promotes the Growth of CD3-CD4-CD8-CD44+CD25+/- Precursor Cells and Blocks the Differentiation Pathway of TCR-alpha Beta Cells in Fetal Thymus Organ Culture. J Immunol. 1993 Apr 1;150(7):2706-16. PubMed PMID: 8095954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous IL-7 promotes the growth of CD3-CD4-CD8-CD44+CD25+/- precursor cells and blocks the differentiation pathway of TCR-alpha beta cells in fetal thymus organ culture. AU - Plum,J, AU - De Smedt,M, AU - Leclercq,G, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 2706 EP - 16 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 150 IS - 7 N2 - Addition of human rIL-7 to fetal thymic organ culture started at day 13, 14, or 15 did not influence the number of cells generated during a 12-day culture period. However, the IL-7 treatment resulted in a preferential expansion of cells with a phenotype characteristic for cells at an early step of differentiation. The cells were CD4-CD8-CD3-CD2- and SCA-1+. Analysis of the coordinate expression of CD44 and CD25 on these cells showed that the majority of the cells were either CD44+CD25- or CD44+CD25intermediate. TCR-alpha beta cells were present but in a significantly lower number as compared to the control cultures. The cell number of TCR-gamma delta cells was increased. All these effects were moderate after 6 days, but unequivocal after 12 days of culture. Treatment of the fetal organ culture with mAb-neutralizing murine IL-7 resulted in an inhibition of the proliferation of the fetal thymocytes. No particular subset studied was preferentially inhibited. By using a model of reconstitution of 14-day embryonic thymuses depleted of thymocytes by deoxyguanosine and reconstituted with fetal day 13 liver cells and set up in organ culture with or without IL-7, it was shown in a clear cut way that IL-7 indeed promotes expansion of the early precursor cells and TCR-gamma delta cells, but prevents the generation of TCR-alpha beta cells. In addition, reconstitution experiments were set up in the presence of mAb-neutralizing murine IL-7. This treatment resulted in the inhibition of the growth of the fetal thymocytes without inhibiting preferentially a particular subset. These data indicate that IL-7 acts at an early step of T cell differentiation and plays a role to expand precursor cells, but prevents this population from additional differentiation towards the TCR-alpha beta pathways, whereas the differentiation towards TCR-gamma delta cells is not influenced or even enhanced. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8095954/Exogenous_IL_7_promotes_the_growth_of_CD3_CD4_CD8_CD44+CD25+/__precursor_cells_and_blocks_the_differentiation_pathway_of_TCR_alpha_beta_cells_in_fetal_thymus_organ_culture_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=8095954 DB - PRIME DP - Unbound Medicine ER -