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EMIT-d.a.u. monoclonal amphetamine/methamphetamine assay. II. Detection of methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA).
Forensic Sci Int. 1993 Apr; 59(1):63-70.FS

Abstract

The cross-reactivity, stereoselectivity and clinical performance of the EMIT-d.a.u. monoclonal amphetamine/methamphetamine immunoassay (EM) for the detection of methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA) in urine was evaluated. The cut-off concentrations of racemic MDA and MDMA were found to be approximately 800 ng/ml and 3000 ng/ml, respectively. The EM assay demonstrated a high selectivity for the S(+) isomer of both MDA and MDMA. Urines collected over a 24-h period from rats administered 20 mg i.v. racemic MDMA were all positive when analyzed by the EM assay. The EM was found vastly superior to the EMIT d.a.u. polyclonal amphetamine/methamphetamine assay for the detection of MDA and MDMA. The EM assay displayed sufficient sensitivity for detection of these drugs following clinical intoxication.

Authors+Show Affiliations

Department of Pathology, Medical College of Virginia Hospitals, Virginia Commonwealth University, Richmond 23298-0597.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8099337

Citation

Poklis, A, et al. "EMIT-d.a.u. Monoclonal Amphetamine/methamphetamine Assay. II. Detection of Methylenedioxyamphetamine (MDA) and Methylenedioxymethamphetamine (MDMA)." Forensic Science International, vol. 59, no. 1, 1993, pp. 63-70.
Poklis A, Fitzgerald RL, Hall KV, et al. EMIT-d.a.u. monoclonal amphetamine/methamphetamine assay. II. Detection of methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA). Forensic Sci Int. 1993;59(1):63-70.
Poklis, A., Fitzgerald, R. L., Hall, K. V., & Saady, J. J. (1993). EMIT-d.a.u. monoclonal amphetamine/methamphetamine assay. II. Detection of methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA). Forensic Science International, 59(1), 63-70.
Poklis A, et al. EMIT-d.a.u. Monoclonal Amphetamine/methamphetamine Assay. II. Detection of Methylenedioxyamphetamine (MDA) and Methylenedioxymethamphetamine (MDMA). Forensic Sci Int. 1993;59(1):63-70. PubMed PMID: 8099337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EMIT-d.a.u. monoclonal amphetamine/methamphetamine assay. II. Detection of methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA). AU - Poklis,A, AU - Fitzgerald,R L, AU - Hall,K V, AU - Saady,J J, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 63 EP - 70 JF - Forensic science international JO - Forensic Sci Int VL - 59 IS - 1 N2 - The cross-reactivity, stereoselectivity and clinical performance of the EMIT-d.a.u. monoclonal amphetamine/methamphetamine immunoassay (EM) for the detection of methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA) in urine was evaluated. The cut-off concentrations of racemic MDA and MDMA were found to be approximately 800 ng/ml and 3000 ng/ml, respectively. The EM assay demonstrated a high selectivity for the S(+) isomer of both MDA and MDMA. Urines collected over a 24-h period from rats administered 20 mg i.v. racemic MDMA were all positive when analyzed by the EM assay. The EM was found vastly superior to the EMIT d.a.u. polyclonal amphetamine/methamphetamine assay for the detection of MDA and MDMA. The EM assay displayed sufficient sensitivity for detection of these drugs following clinical intoxication. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/8099337/EMIT_d_a_u__monoclonal_amphetamine/methamphetamine_assay__II__Detection_of_methylenedioxyamphetamine__MDA__and_methylenedioxymethamphetamine__MDMA__ L2 - https://linkinghub.elsevier.com/retrieve/pii/0379-0738(93)90312-X DB - PRIME DP - Unbound Medicine ER -