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The leukocyte function-associated antigen-1 (LFA-1) is involved in the interferon-alpha response induced by herpes simplex virus in blood leukocytes.
J Interferon Res 1993; 13(3):203-8JI

Abstract

The role of the leukocyte function-associated antigen-1 (LFA-1) family of integrins (beta 2 integrins) in the interferon-alpha (IFN-alpha) response was examined, using human peripheral blood mononuclear cells (PBMCs) stimulated in vitro by glutaraldehyde-fixed Herpes simplex virus-infected WISH amnion cells. Monoclonal antibodies (mAbs) to the beta 2 chain (CD18) and to the alpha chain of LFA-1 (CD11a) reduced the number of IFN-alpha-producing cells (IPCs) by 30-50%, but mAbs to CD11b or c caused no inhibition. The IB4 mAb to CD18 was inhibitory when added during the first 2 h of the IFN-alpha response, but did not alter its kinetic. In contrast, the IB4 prevented the early enhancement of the IFN-alpha response caused by addition of interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). However, a delayed down-regulation of the IPC response occurred in such PBMC cultures, and a paradoxical increase in the total production of IFN-alpha. The results suggest that LFA-1 (CD11a/CD18) participates in the early phase of the IFN-alpha response and may be activated by cytokines such as IL-3 and GM-CSF.

Authors+Show Affiliations

Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8103535

Citation

Cederblad, B, et al. "The Leukocyte Function-associated Antigen-1 (LFA-1) Is Involved in the Interferon-alpha Response Induced By Herpes Simplex Virus in Blood Leukocytes." Journal of Interferon Research, vol. 13, no. 3, 1993, pp. 203-8.
Cederblad B, Sandberg K, Alm GV. The leukocyte function-associated antigen-1 (LFA-1) is involved in the interferon-alpha response induced by herpes simplex virus in blood leukocytes. J Interferon Res. 1993;13(3):203-8.
Cederblad, B., Sandberg, K., & Alm, G. V. (1993). The leukocyte function-associated antigen-1 (LFA-1) is involved in the interferon-alpha response induced by herpes simplex virus in blood leukocytes. Journal of Interferon Research, 13(3), pp. 203-8.
Cederblad B, Sandberg K, Alm GV. The Leukocyte Function-associated Antigen-1 (LFA-1) Is Involved in the Interferon-alpha Response Induced By Herpes Simplex Virus in Blood Leukocytes. J Interferon Res. 1993;13(3):203-8. PubMed PMID: 8103535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The leukocyte function-associated antigen-1 (LFA-1) is involved in the interferon-alpha response induced by herpes simplex virus in blood leukocytes. AU - Cederblad,B, AU - Sandberg,K, AU - Alm,G V, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 203 EP - 8 JF - Journal of interferon research JO - J. Interferon Res. VL - 13 IS - 3 N2 - The role of the leukocyte function-associated antigen-1 (LFA-1) family of integrins (beta 2 integrins) in the interferon-alpha (IFN-alpha) response was examined, using human peripheral blood mononuclear cells (PBMCs) stimulated in vitro by glutaraldehyde-fixed Herpes simplex virus-infected WISH amnion cells. Monoclonal antibodies (mAbs) to the beta 2 chain (CD18) and to the alpha chain of LFA-1 (CD11a) reduced the number of IFN-alpha-producing cells (IPCs) by 30-50%, but mAbs to CD11b or c caused no inhibition. The IB4 mAb to CD18 was inhibitory when added during the first 2 h of the IFN-alpha response, but did not alter its kinetic. In contrast, the IB4 prevented the early enhancement of the IFN-alpha response caused by addition of interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). However, a delayed down-regulation of the IPC response occurred in such PBMC cultures, and a paradoxical increase in the total production of IFN-alpha. The results suggest that LFA-1 (CD11a/CD18) participates in the early phase of the IFN-alpha response and may be activated by cytokines such as IL-3 and GM-CSF. SN - 0197-8357 UR - https://www.unboundmedicine.com/medline/citation/8103535/The_leukocyte_function_associated_antigen_1__LFA_1__is_involved_in_the_interferon_alpha_response_induced_by_herpes_simplex_virus_in_blood_leukocytes_ DB - PRIME DP - Unbound Medicine ER -