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Distribution and phenotypes of duodenal intraepithelial gamma/delta T cells in patients with various types of primary B-cell deficiency.
Clin Immunol Immunopathol. 1993 Sep; 68(3):301-10.CI

Abstract

Expression of the gamma/delta T-cell receptor (TCR) on CD3+ intraepithelial lymphocytes (IEL) was studied in situ by two-color immunofluorescence on duodenal tissue sections from 34 infection-prone, adult patients with various types of primary hypogammaglobulinemia, classical Bruton's, Bruton-like or congenital, and common variable immunodeficiency. TCR gamma/delta+ IEL proportions (median 4.3%, range 0.3-43.3%) were within the range (0.3-38.3%) for histologically normal controls (n = 11), and there was no significant difference between the three patient categories. The total number of CD3+ IEL (mostly CD8+) per intestinal length unit was significantly higher (P < 0.004) in patients than in controls. In addition, TCR gamma/delta+ IEL per length unit, as well as TCR gamma/delta+ IEL proportions, were significantly increased (P < 0.008 and P < 0.05) in 14 patients with intestinal villous atrophy. Paired staining revealed that most (approximately 94%) TCR gamma/delta+ IEL in B-cell deficiency were CD8-, and a large fraction (approximately 67%) expressed the V delta 1/J delta 1-encoded epitope. No relationship was found between CD3+ or TCR gamma/delta+ IEL and the number of CD3+, CD4+, CD8+, and B lymphocytes or the CD4:CD8 ratio in peripheral blood. TCR gamma/delta+ IEL thus appeared to maintain a normal distribution in B-cell deficiency, except for being increased in patients with villous atrophy. Enhanced T-cell-mediated immunity, as possibly reflected by the numerical increase of CD3+ IEL, might compensate for a deficient mucosal B-cell system.

Authors+Show Affiliations

Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), University of Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8103721

Citation

Nilssen, D E., et al. "Distribution and Phenotypes of Duodenal Intraepithelial Gamma/delta T Cells in Patients With Various Types of Primary B-cell Deficiency." Clinical Immunology and Immunopathology, vol. 68, no. 3, 1993, pp. 301-10.
Nilssen DE, Halstensen TS, Frøland SS, et al. Distribution and phenotypes of duodenal intraepithelial gamma/delta T cells in patients with various types of primary B-cell deficiency. Clin Immunol Immunopathol. 1993;68(3):301-10.
Nilssen, D. E., Halstensen, T. S., Frøland, S. S., Fausa, O., & Brandtzaeg, P. (1993). Distribution and phenotypes of duodenal intraepithelial gamma/delta T cells in patients with various types of primary B-cell deficiency. Clinical Immunology and Immunopathology, 68(3), 301-10.
Nilssen DE, et al. Distribution and Phenotypes of Duodenal Intraepithelial Gamma/delta T Cells in Patients With Various Types of Primary B-cell Deficiency. Clin Immunol Immunopathol. 1993;68(3):301-10. PubMed PMID: 8103721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution and phenotypes of duodenal intraepithelial gamma/delta T cells in patients with various types of primary B-cell deficiency. AU - Nilssen,D E, AU - Halstensen,T S, AU - Frøland,S S, AU - Fausa,O, AU - Brandtzaeg,P, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 301 EP - 10 JF - Clinical immunology and immunopathology JO - Clin Immunol Immunopathol VL - 68 IS - 3 N2 - Expression of the gamma/delta T-cell receptor (TCR) on CD3+ intraepithelial lymphocytes (IEL) was studied in situ by two-color immunofluorescence on duodenal tissue sections from 34 infection-prone, adult patients with various types of primary hypogammaglobulinemia, classical Bruton's, Bruton-like or congenital, and common variable immunodeficiency. TCR gamma/delta+ IEL proportions (median 4.3%, range 0.3-43.3%) were within the range (0.3-38.3%) for histologically normal controls (n = 11), and there was no significant difference between the three patient categories. The total number of CD3+ IEL (mostly CD8+) per intestinal length unit was significantly higher (P < 0.004) in patients than in controls. In addition, TCR gamma/delta+ IEL per length unit, as well as TCR gamma/delta+ IEL proportions, were significantly increased (P < 0.008 and P < 0.05) in 14 patients with intestinal villous atrophy. Paired staining revealed that most (approximately 94%) TCR gamma/delta+ IEL in B-cell deficiency were CD8-, and a large fraction (approximately 67%) expressed the V delta 1/J delta 1-encoded epitope. No relationship was found between CD3+ or TCR gamma/delta+ IEL and the number of CD3+, CD4+, CD8+, and B lymphocytes or the CD4:CD8 ratio in peripheral blood. TCR gamma/delta+ IEL thus appeared to maintain a normal distribution in B-cell deficiency, except for being increased in patients with villous atrophy. Enhanced T-cell-mediated immunity, as possibly reflected by the numerical increase of CD3+ IEL, might compensate for a deficient mucosal B-cell system. SN - 0090-1229 UR - https://www.unboundmedicine.com/medline/citation/8103721/Distribution_and_phenotypes_of_duodenal_intraepithelial_gamma/delta_T_cells_in_patients_with_various_types_of_primary_B_cell_deficiency_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090122983711311 DB - PRIME DP - Unbound Medicine ER -