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Cytokine expression by neutrophils and macrophages in vivo: endotoxin induces tumor necrosis factor-alpha, macrophage inflammatory protein-2, interleukin-1 beta, and interleukin-6 but not RANTES or transforming growth factor-beta 1 mRNA expression in acute lung inflammation.
Am J Respir Cell Mol Biol. 1994 Feb; 10(2):148-53.AJ

Abstract

Using a rat model of acute lung inflammation induced by intratracheal instillation of lipopolysaccharide (LPS), we investigated the kinetics of mRNA expression and the potential cellular sources of tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-2 (MIP-2), interleukin (IL)-1 beta, IL-6, RANTES, and transforming growth factor-beta 1 (TGF-beta 1). By Northern blot analysis, TNF-alpha and MIP-2 mRNAs in total lung tissue increased markedly by 30 min and peaked by 1 h after LPS exposure, whereas expression of IL-1 beta and IL-6 was not detected until 1 h and peaked within 6 h. In contrast, neither RANTES nor TGF-beta 1 mRNA was induced by LPS throughout 72 h, although a basal expression was detected in both saline- and LPS-treated lung tissues. At 1 h after LPS, the bronchoalveolar lavage (BAL) fluid contained about 98% alveolar macrophages (AM), whereas by 6 or 12 h, 88% of BAL cells were polymorphonuclear neutrophils (PMN). Upon extraction of total RNA after separation of AM from PMN in BAL, Northern analysis showed that at 1 h, AM expressed pronounced signals for TNF-alpha, MIP-2, IL-1 beta, and IL-6. At 6 and 12 h, however, while cytokine transcripts decreased in AM, PMN exhibited strong signals for these cytokines. A low basal noninducible signal for TGF-beta 1 but not RANTES was detected in both AM and PMN. Finally, by in situ hybridization techniques, PMN in the lung tissue, particularly those located in the vicinity of the bronchiole and vasculature, were demonstrated to localize MIP-2 mRNA.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Pathology, McMaster University, Hamilton, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8110470

Citation

Xing, Z, et al. "Cytokine Expression By Neutrophils and Macrophages in Vivo: Endotoxin Induces Tumor Necrosis Factor-alpha, Macrophage Inflammatory Protein-2, Interleukin-1 Beta, and Interleukin-6 but Not RANTES or Transforming Growth Factor-beta 1 mRNA Expression in Acute Lung Inflammation." American Journal of Respiratory Cell and Molecular Biology, vol. 10, no. 2, 1994, pp. 148-53.
Xing Z, Jordana M, Kirpalani H, et al. Cytokine expression by neutrophils and macrophages in vivo: endotoxin induces tumor necrosis factor-alpha, macrophage inflammatory protein-2, interleukin-1 beta, and interleukin-6 but not RANTES or transforming growth factor-beta 1 mRNA expression in acute lung inflammation. Am J Respir Cell Mol Biol. 1994;10(2):148-53.
Xing, Z., Jordana, M., Kirpalani, H., Driscoll, K. E., Schall, T. J., & Gauldie, J. (1994). Cytokine expression by neutrophils and macrophages in vivo: endotoxin induces tumor necrosis factor-alpha, macrophage inflammatory protein-2, interleukin-1 beta, and interleukin-6 but not RANTES or transforming growth factor-beta 1 mRNA expression in acute lung inflammation. American Journal of Respiratory Cell and Molecular Biology, 10(2), 148-53.
Xing Z, et al. Cytokine Expression By Neutrophils and Macrophages in Vivo: Endotoxin Induces Tumor Necrosis Factor-alpha, Macrophage Inflammatory Protein-2, Interleukin-1 Beta, and Interleukin-6 but Not RANTES or Transforming Growth Factor-beta 1 mRNA Expression in Acute Lung Inflammation. Am J Respir Cell Mol Biol. 1994;10(2):148-53. PubMed PMID: 8110470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine expression by neutrophils and macrophages in vivo: endotoxin induces tumor necrosis factor-alpha, macrophage inflammatory protein-2, interleukin-1 beta, and interleukin-6 but not RANTES or transforming growth factor-beta 1 mRNA expression in acute lung inflammation. AU - Xing,Z, AU - Jordana,M, AU - Kirpalani,H, AU - Driscoll,K E, AU - Schall,T J, AU - Gauldie,J, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 148 EP - 53 JF - American journal of respiratory cell and molecular biology JO - Am J Respir Cell Mol Biol VL - 10 IS - 2 N2 - Using a rat model of acute lung inflammation induced by intratracheal instillation of lipopolysaccharide (LPS), we investigated the kinetics of mRNA expression and the potential cellular sources of tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-2 (MIP-2), interleukin (IL)-1 beta, IL-6, RANTES, and transforming growth factor-beta 1 (TGF-beta 1). By Northern blot analysis, TNF-alpha and MIP-2 mRNAs in total lung tissue increased markedly by 30 min and peaked by 1 h after LPS exposure, whereas expression of IL-1 beta and IL-6 was not detected until 1 h and peaked within 6 h. In contrast, neither RANTES nor TGF-beta 1 mRNA was induced by LPS throughout 72 h, although a basal expression was detected in both saline- and LPS-treated lung tissues. At 1 h after LPS, the bronchoalveolar lavage (BAL) fluid contained about 98% alveolar macrophages (AM), whereas by 6 or 12 h, 88% of BAL cells were polymorphonuclear neutrophils (PMN). Upon extraction of total RNA after separation of AM from PMN in BAL, Northern analysis showed that at 1 h, AM expressed pronounced signals for TNF-alpha, MIP-2, IL-1 beta, and IL-6. At 6 and 12 h, however, while cytokine transcripts decreased in AM, PMN exhibited strong signals for these cytokines. A low basal noninducible signal for TGF-beta 1 but not RANTES was detected in both AM and PMN. Finally, by in situ hybridization techniques, PMN in the lung tissue, particularly those located in the vicinity of the bronchiole and vasculature, were demonstrated to localize MIP-2 mRNA.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 1044-1549 UR - https://www.unboundmedicine.com/medline/citation/8110470/Cytokine_expression_by_neutrophils_and_macrophages_in_vivo:_endotoxin_induces_tumor_necrosis_factor_alpha_macrophage_inflammatory_protein_2_interleukin_1_beta_and_interleukin_6_but_not_RANTES_or_transforming_growth_factor_beta_1_mRNA_expression_in_acute_lung_inflammation_ L2 - https://www.atsjournals.org/doi/10.1165/ajrcmb.10.2.8110470?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -