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Tumor necrosis factor receptor p75 mediates cell-specific activation of nuclear factor kappa B and induction of human cytomegalovirus enhancer.
J Biol Chem. 1994 Mar 11; 269(10):7785-91.JB

Abstract

The functional role of human tumor necrosis factor receptor (TNFR) p75 was studied by the use of TNFR p75-specific agonistic antibodies. Human SW480T adenocarcinoma cells, stably transfected with a reporter construct containing beta-galactosidase under the control of human cytomegalovirus immediate early enhancer, were stimulated with anti-TNFR p75 polyclonal antiserum or monoclonal antibodies followed by measurement of beta-galactosidase activity and analysis by electrophoretic mobility shift assays. It was found that cross-linking of TNFR p75 led to strong induction of the human cytomegalovirus enhancer as well as activation of nuclear factor-kappa B (NF-kappa B). Stimulation of TNFR p75 also mediated activation of NF-kappa B in human KYM-1 rhabdomyosarcoma cells but not in other cell types such as U937 and HL-60 monocytic cells or in Eahy 926 endothelial cells. NF-kappa B activation induced by TNFR p75 was delayed approximately 15 min compared with NF-kappa B activation induced by TNFR p55, indicating that the two TNFRs activate NF-kappa B through different signaling pathways. The data presented in this study identify intracellular responses mediated by TNFR p75 which have not been reported previously and suggest that TNFR p75-induced activation of NF-kappa B is strictly cell type-specific.

Authors+Show Affiliations

Institute of Cancer Research, University of Trondheim, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8126005

Citation

Laegreid, A, et al. "Tumor Necrosis Factor Receptor P75 Mediates Cell-specific Activation of Nuclear Factor Kappa B and Induction of Human Cytomegalovirus Enhancer." The Journal of Biological Chemistry, vol. 269, no. 10, 1994, pp. 7785-91.
Laegreid A, Medvedev A, Nonstad U, et al. Tumor necrosis factor receptor p75 mediates cell-specific activation of nuclear factor kappa B and induction of human cytomegalovirus enhancer. J Biol Chem. 1994;269(10):7785-91.
Laegreid, A., Medvedev, A., Nonstad, U., Bombara, M. P., Ranges, G., Sundan, A., & Espevik, T. (1994). Tumor necrosis factor receptor p75 mediates cell-specific activation of nuclear factor kappa B and induction of human cytomegalovirus enhancer. The Journal of Biological Chemistry, 269(10), 7785-91.
Laegreid A, et al. Tumor Necrosis Factor Receptor P75 Mediates Cell-specific Activation of Nuclear Factor Kappa B and Induction of Human Cytomegalovirus Enhancer. J Biol Chem. 1994 Mar 11;269(10):7785-91. PubMed PMID: 8126005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tumor necrosis factor receptor p75 mediates cell-specific activation of nuclear factor kappa B and induction of human cytomegalovirus enhancer. AU - Laegreid,A, AU - Medvedev,A, AU - Nonstad,U, AU - Bombara,M P, AU - Ranges,G, AU - Sundan,A, AU - Espevik,T, PY - 1994/3/11/pubmed PY - 1994/3/11/medline PY - 1994/3/11/entrez SP - 7785 EP - 91 JF - The Journal of biological chemistry JO - J Biol Chem VL - 269 IS - 10 N2 - The functional role of human tumor necrosis factor receptor (TNFR) p75 was studied by the use of TNFR p75-specific agonistic antibodies. Human SW480T adenocarcinoma cells, stably transfected with a reporter construct containing beta-galactosidase under the control of human cytomegalovirus immediate early enhancer, were stimulated with anti-TNFR p75 polyclonal antiserum or monoclonal antibodies followed by measurement of beta-galactosidase activity and analysis by electrophoretic mobility shift assays. It was found that cross-linking of TNFR p75 led to strong induction of the human cytomegalovirus enhancer as well as activation of nuclear factor-kappa B (NF-kappa B). Stimulation of TNFR p75 also mediated activation of NF-kappa B in human KYM-1 rhabdomyosarcoma cells but not in other cell types such as U937 and HL-60 monocytic cells or in Eahy 926 endothelial cells. NF-kappa B activation induced by TNFR p75 was delayed approximately 15 min compared with NF-kappa B activation induced by TNFR p55, indicating that the two TNFRs activate NF-kappa B through different signaling pathways. The data presented in this study identify intracellular responses mediated by TNFR p75 which have not been reported previously and suggest that TNFR p75-induced activation of NF-kappa B is strictly cell type-specific. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/8126005/Tumor_necrosis_factor_receptor_p75_mediates_cell_specific_activation_of_nuclear_factor_kappa_B_and_induction_of_human_cytomegalovirus_enhancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(17)37355-6 DB - PRIME DP - Unbound Medicine ER -