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Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection.
Clin Chem 1994; 40(3):411-6CC

Abstract

We describe the use of HPLC with multiwavelength detection to measure retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, beta-carotene, and the linoleate, oleate, palmitate, and stearate esters of retinol in a single 200-microL serum sample. The method is sensitive enough to detect individual retinyl esters in fasting serum from a nonhyperlipidemic population and requires only 12 min for each sample. Serum concentration ranges and means are reported for retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and the sum of the retinyl esters from serum analyses of 3480 participants from several different studies.

Authors+Show Affiliations

Division of Environmental Health Laboratory Sciences, Centers for Disease Control and Prevention, Public Health Service, Atlanta, GA 30341-3724.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8131277

Citation

Sowell, A L., et al. "Retinol, Alpha-tocopherol, Lutein/zeaxanthin, Beta-cryptoxanthin, Lycopene, Alpha-carotene, Trans-beta-carotene, and Four Retinyl Esters in Serum Determined Simultaneously By Reversed-phase HPLC With Multiwavelength Detection." Clinical Chemistry, vol. 40, no. 3, 1994, pp. 411-6.
Sowell AL, Huff DL, Yeager PR, et al. Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection. Clin Chem. 1994;40(3):411-6.
Sowell, A. L., Huff, D. L., Yeager, P. R., Caudill, S. P., & Gunter, E. W. (1994). Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection. Clinical Chemistry, 40(3), pp. 411-6.
Sowell AL, et al. Retinol, Alpha-tocopherol, Lutein/zeaxanthin, Beta-cryptoxanthin, Lycopene, Alpha-carotene, Trans-beta-carotene, and Four Retinyl Esters in Serum Determined Simultaneously By Reversed-phase HPLC With Multiwavelength Detection. Clin Chem. 1994;40(3):411-6. PubMed PMID: 8131277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection. AU - Sowell,A L, AU - Huff,D L, AU - Yeager,P R, AU - Caudill,S P, AU - Gunter,E W, PY - 1994/3/1/pubmed PY - 1994/3/1/medline PY - 1994/3/1/entrez SP - 411 EP - 6 JF - Clinical chemistry JO - Clin. Chem. VL - 40 IS - 3 N2 - We describe the use of HPLC with multiwavelength detection to measure retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, beta-carotene, and the linoleate, oleate, palmitate, and stearate esters of retinol in a single 200-microL serum sample. The method is sensitive enough to detect individual retinyl esters in fasting serum from a nonhyperlipidemic population and requires only 12 min for each sample. Serum concentration ranges and means are reported for retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and the sum of the retinyl esters from serum analyses of 3480 participants from several different studies. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/8131277/Retinol_alpha_tocopherol_lutein/zeaxanthin_beta_cryptoxanthin_lycopene_alpha_carotene_trans_beta_carotene_and_four_retinyl_esters_in_serum_determined_simultaneously_by_reversed_phase_HPLC_with_multiwavelength_detection_ L2 - http://www.clinchem.org/cgi/pmidlookup?view=long&pmid=8131277 DB - PRIME DP - Unbound Medicine ER -