Tags

Type your tag names separated by a space and hit enter

[Supercopy plasmid based on the replicon from the temperate N15 bacteriophage].

Abstract

Mini-plasmids, based on the N15 temperate bacteriophage replicon, are described. One of these, N15-203 linear 13.8 kb plasmid, has anomalously high copy number--more than 250 per one bacterial chromosome and the amount of plasmid DNA comprises about half of the total DNA of a cell. This property of N15-203 plasmid is realized only in the strain lysogenic for a N15 phage and is lost for the circular deletion versions of N15-203. The efficiency of transformation of E. coli C (N15) strain is essentially the same for N15-203 and pUC4K plasmids. Insertion of foreign DNA with a size up to 20 kb into BgIII cloning site of N15-203 plasmid does not decrease significantly efficiency of transformation calculated per number of DNA molecules and the total amount of plasmid DNA in a cell. N15-203 plasmid may be used as a vector for molecular cloning of relatively large DNA fragments, and in those biotechnology processes when productivity depends on a vector's copy number.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

8133851

Citation

Ravin, N V., and V K. Ravin. "[Supercopy Plasmid Based On the Replicon From the Temperate N15 Bacteriophage]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1994, pp. 37-9.
Ravin NV, Ravin VK. [Supercopy plasmid based on the replicon from the temperate N15 bacteriophage]. Mol Gen Mikrobiol Virusol. 1994.
Ravin, N. V., & Ravin, V. K. (1994). [Supercopy plasmid based on the replicon from the temperate N15 bacteriophage]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (1), 37-9.
Ravin NV, Ravin VK. [Supercopy Plasmid Based On the Replicon From the Temperate N15 Bacteriophage]. Mol Gen Mikrobiol Virusol. 1994 Jan-Feb;(1)37-9. PubMed PMID: 8133851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Supercopy plasmid based on the replicon from the temperate N15 bacteriophage]. AU - Ravin,N V, AU - Ravin,V K, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 37 EP - 9 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol. Gen. Mikrobiol. Virusol. IS - 1 N2 - Mini-plasmids, based on the N15 temperate bacteriophage replicon, are described. One of these, N15-203 linear 13.8 kb plasmid, has anomalously high copy number--more than 250 per one bacterial chromosome and the amount of plasmid DNA comprises about half of the total DNA of a cell. This property of N15-203 plasmid is realized only in the strain lysogenic for a N15 phage and is lost for the circular deletion versions of N15-203. The efficiency of transformation of E. coli C (N15) strain is essentially the same for N15-203 and pUC4K plasmids. Insertion of foreign DNA with a size up to 20 kb into BgIII cloning site of N15-203 plasmid does not decrease significantly efficiency of transformation calculated per number of DNA molecules and the total amount of plasmid DNA in a cell. N15-203 plasmid may be used as a vector for molecular cloning of relatively large DNA fragments, and in those biotechnology processes when productivity depends on a vector's copy number. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/8133851/[Supercopy_plasmid_based_on_the_replicon_from_the_temperate_N15_bacteriophage]_ DB - PRIME DP - Unbound Medicine ER -