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Synaptic plasma membrane structure and polarity of long-sleep and short-sleep mice.
Arch Biochem Biophys. 1994 Mar; 309(2):369-76.AB

Abstract

Membrane dielectric as a primary basis for effects of ethanol was examined in synaptic plasma membranes (SPM) of genetically selected ethanol-sensitive long-sleep (LS) and ethanol-resistant short-sleep (SS) mice. Multifrequency phase and modulation of fluorometry of diphenylhexatriene (DPH) was used to resolve structural and dielectric differences in the membrane interior core. Fluorescence spectral peak ratios, fluorescence lifetime analysis, and initial rates of photoreaction of DPH in SPM provided sensitive measures of SPM interior core dielectric properties. The membrane microenvironment sensed by DPH was more polar in SPM from SS mice than in SPM from LS mice. Physiological concentrations of ethanol in vitro (25-75 mM) increased the SPM interior core dielectric and potentiated photoreaction of DPH with other membrane components of SPM from LS, but not SS, mice. These effects of ethanol in vitro were maximal by 75 mM ethanol and/or exacerbated at higher ethanol. In addition, ethanol in vitro increased the fraction of DPH associated with photoreaction products with lipids from SPM of ethanol-sensitive LS mice. The data were consistent with ethanol in vitro increasing the polar molecules (ethanol and/or water) of SPM from LS but not SS mice. It is suggested that ethanol alters the polarity and increases reactivity of the interior core lipid-protein interface.

Authors+Show Affiliations

Department of Pharmacology and Cell Biophysics, University of Cincinnati, Ohio 45267-0004.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8135550

Citation

Schroeder, F, et al. "Synaptic Plasma Membrane Structure and Polarity of Long-sleep and Short-sleep Mice." Archives of Biochemistry and Biophysics, vol. 309, no. 2, 1994, pp. 369-76.
Schroeder F, Colles SM, Kreishman GP, et al. Synaptic plasma membrane structure and polarity of long-sleep and short-sleep mice. Arch Biochem Biophys. 1994;309(2):369-76.
Schroeder, F., Colles, S. M., Kreishman, G. P., Heyliger, C. E., & Wood, W. G. (1994). Synaptic plasma membrane structure and polarity of long-sleep and short-sleep mice. Archives of Biochemistry and Biophysics, 309(2), 369-76.
Schroeder F, et al. Synaptic Plasma Membrane Structure and Polarity of Long-sleep and Short-sleep Mice. Arch Biochem Biophys. 1994;309(2):369-76. PubMed PMID: 8135550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synaptic plasma membrane structure and polarity of long-sleep and short-sleep mice. AU - Schroeder,F, AU - Colles,S M, AU - Kreishman,G P, AU - Heyliger,C E, AU - Wood,W G, PY - 1994/3/1/pubmed PY - 1994/3/1/medline PY - 1994/3/1/entrez SP - 369 EP - 76 JF - Archives of biochemistry and biophysics JO - Arch Biochem Biophys VL - 309 IS - 2 N2 - Membrane dielectric as a primary basis for effects of ethanol was examined in synaptic plasma membranes (SPM) of genetically selected ethanol-sensitive long-sleep (LS) and ethanol-resistant short-sleep (SS) mice. Multifrequency phase and modulation of fluorometry of diphenylhexatriene (DPH) was used to resolve structural and dielectric differences in the membrane interior core. Fluorescence spectral peak ratios, fluorescence lifetime analysis, and initial rates of photoreaction of DPH in SPM provided sensitive measures of SPM interior core dielectric properties. The membrane microenvironment sensed by DPH was more polar in SPM from SS mice than in SPM from LS mice. Physiological concentrations of ethanol in vitro (25-75 mM) increased the SPM interior core dielectric and potentiated photoreaction of DPH with other membrane components of SPM from LS, but not SS, mice. These effects of ethanol in vitro were maximal by 75 mM ethanol and/or exacerbated at higher ethanol. In addition, ethanol in vitro increased the fraction of DPH associated with photoreaction products with lipids from SPM of ethanol-sensitive LS mice. The data were consistent with ethanol in vitro increasing the polar molecules (ethanol and/or water) of SPM from LS but not SS mice. It is suggested that ethanol alters the polarity and increases reactivity of the interior core lipid-protein interface. SN - 0003-9861 UR - https://www.unboundmedicine.com/medline/citation/8135550/Synaptic_plasma_membrane_structure_and_polarity_of_long_sleep_and_short_sleep_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9861(84)71126-X DB - PRIME DP - Unbound Medicine ER -