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Ethanol modulates [125I]calmodulin binding to synaptic plasma membranes from rat brain.
J Pharmacol Exp Ther. 1994 Mar; 268(3):1183-9.JP

Abstract

The effects of ethanol in vitro on Ca(++)-dependent binding of [125I] calmodulin to brain synaptic plasma membranes (SPM) from control and chronically ethanol-treated rats were studied. In SPM from control animals, ethanol at 50 to 200 mM inhibited [125I] calmodulin binding; the inhibition was correlated with a decreased membrane affinity for [125I]calmodulin as shown by Scatchard analysis, and an increased dissociation of [125I]calmodulin-membrane complexes as shown by kinetic analysis. Arrhenius analysis indicates that [125I]calmodulin binding was influenced by lipid transition of the membrane, and that ethanol in vitro resulted in a shift of the transition temperature toward a lower value. From animals receiving chronic ethanol treatment (3 weeks), the SPM were found to be resistant to the inhibitory effect of ethanol on binding. The resistance to ethanol inhibition was correlated with a higher membrane affinity for [125I]calmodulin and a higher transition temperature, as compared with control SPM. Because a variety of membrane-bound processes are regulated by calmodulin or calmodulin-dependent processes, the inhibitory effect of ethanol on membrane binding of calmodulin could lead to a cascade of consequences in synaptic function. Moreover, the resistance of the membranes to ethanol inhibition after chronic ethanol treatment implies that membrane binding of calmodulin is part of the mechanism underlying alcohol tolerance and dependence.

Authors+Show Affiliations

Department of Pharmacology and Molecular Biology, Chicago Medical School, Illinois.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8138930

Citation

Sze, P Y., and Z Iqbal. "Ethanol Modulates [125I]calmodulin Binding to Synaptic Plasma Membranes From Rat Brain." The Journal of Pharmacology and Experimental Therapeutics, vol. 268, no. 3, 1994, pp. 1183-9.
Sze PY, Iqbal Z. Ethanol modulates [125I]calmodulin binding to synaptic plasma membranes from rat brain. J Pharmacol Exp Ther. 1994;268(3):1183-9.
Sze, P. Y., & Iqbal, Z. (1994). Ethanol modulates [125I]calmodulin binding to synaptic plasma membranes from rat brain. The Journal of Pharmacology and Experimental Therapeutics, 268(3), 1183-9.
Sze PY, Iqbal Z. Ethanol Modulates [125I]calmodulin Binding to Synaptic Plasma Membranes From Rat Brain. J Pharmacol Exp Ther. 1994;268(3):1183-9. PubMed PMID: 8138930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethanol modulates [125I]calmodulin binding to synaptic plasma membranes from rat brain. AU - Sze,P Y, AU - Iqbal,Z, PY - 1994/3/1/pubmed PY - 1994/3/1/medline PY - 1994/3/1/entrez SP - 1183 EP - 9 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 268 IS - 3 N2 - The effects of ethanol in vitro on Ca(++)-dependent binding of [125I] calmodulin to brain synaptic plasma membranes (SPM) from control and chronically ethanol-treated rats were studied. In SPM from control animals, ethanol at 50 to 200 mM inhibited [125I] calmodulin binding; the inhibition was correlated with a decreased membrane affinity for [125I]calmodulin as shown by Scatchard analysis, and an increased dissociation of [125I]calmodulin-membrane complexes as shown by kinetic analysis. Arrhenius analysis indicates that [125I]calmodulin binding was influenced by lipid transition of the membrane, and that ethanol in vitro resulted in a shift of the transition temperature toward a lower value. From animals receiving chronic ethanol treatment (3 weeks), the SPM were found to be resistant to the inhibitory effect of ethanol on binding. The resistance to ethanol inhibition was correlated with a higher membrane affinity for [125I]calmodulin and a higher transition temperature, as compared with control SPM. Because a variety of membrane-bound processes are regulated by calmodulin or calmodulin-dependent processes, the inhibitory effect of ethanol on membrane binding of calmodulin could lead to a cascade of consequences in synaptic function. Moreover, the resistance of the membranes to ethanol inhibition after chronic ethanol treatment implies that membrane binding of calmodulin is part of the mechanism underlying alcohol tolerance and dependence. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/8138930/Ethanol_modulates_[125I]calmodulin_binding_to_synaptic_plasma_membranes_from_rat_brain_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=8138930 DB - PRIME DP - Unbound Medicine ER -